Atcc crl 2922

Endothelial EA.hy926 cells (ATCC® CRL‐2922™; ATCC, LGC Standards GmbH) were cultured in DMEM/10% FCS (Thermo Fisher; Biochrom GmbH) at 37°C/5% CO₂. For stimulation experiments, cells were incubated with 50 ng/ml TNF‐α (Thermo Fisher) for 72 hr. Bay 11‐7085 (Cayman Chemical; 10 μM) was used to inhibit NF‐κB activation.

Human endothelial-like cells EA.hy926 (ATCC® CRL-2922™) were obtained from ATCC and tested to exclude Mycoplasma spp. contamination (PromoKine). Cells were maintained and grown in DMEM (PAN Biotech) with 10% fetal bovine serum (FBS) (Bio&Sell) and 1% HAT (Thermo Fisher). A confluence of 80% has been used for all experiments. Furthermore, the cells were prepared under confluent conditions [8 (link)].

The human umbilical vein cell lines (EA.hy926) (ATCC-CRL2922) and the primary umbilical vein endothelial cells (HUVECs) (ATCC PCS-100-013) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). EA.hy926 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin. HUVECs were cultured in vascular basal medium supplemented with endothelial cell growth kit-BBE, and penicillin-streptomycin-amphotericin B solution. Cells were maintained in humidified atmosphere of 95% air and 5% carbon dioxide at 37 °C until 80% confluence. HUVECs of passage 6–10 and EA.hy926 cells of passage 6–15 were used in all experiments.