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Fosl1

Manufactured by Abcam
Sourced in United Kingdom, United States

FOSL1 is a protein-coding gene that produces the FOS-like antigen 1 protein. This protein is a member of the Fos gene family and is involved in regulating the activity of other genes. The FOSL1 gene is expressed in various tissues and plays a role in cellular processes such as proliferation, differentiation, and transformation.

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2 protocols using fosl1

1

FOSL1 Knockdown Modulates Diabetic Progression

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Short hairpin (sh) RNA targeting FOSL1 (sh-FOSL1), sh-negative control (sh-NC), adenovirus vector (Ad)-sh-FOSL1, and Ad-sh-NC were purchased from Sangon Biotech (Shanghai, China). Streptozotocin (STZ) was obtained from Sigma Aldrich (San Luis, MO, USA). The RevertAid H Minus First Strand cDNA Synthesis kit, DyNAmo Flash SYBR Green qPCR kit, and apoptosis detection kit were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). The primary antibodies (FOSL1, p-ERK, ERK, c-fos, c-jun, and GAPDH) and the HRP-conjugated secondary antibody used for western blotting were procured from Abcam (Cambridge, UK).
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2

Protein Expression Profiling

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Primary antibodies against NOL6 (Abcam, Cambridge, UK), CEBPA (CST, Boston, USA), MAP2K5 (Abcam), ID1 (Abcam), MAPK8 (CST), SNAI2 (CST), FOSL1 (Abcam), Flag (Sigma, St. Louis, MO, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Santa Cruz, CA, USA) were used.
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