Luminex 200 machine

Manufactured by Merck Group

The Luminex 200 is a multiplex bead-based assay system used for the detection and quantification of a variety of analytes, including proteins, nucleic acids, and other biomolecules. It utilizes flow cytometry and fluorescent detection technologies to analyze multiple targets simultaneously in a single sample. The core function of the Luminex 200 is to enable high-throughput, multiplexed analysis of biological samples.

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Lab products found in correlation

Ascensia breeze 2 glucometer, by Bayer (4 mentions) Mmhmag 44k, by Merck Group (1 mentions) Ezmadp 60k, by Merck Group (1 mentions) Ezrmfgf21 26k, by Merck Group (1 mentions) Colorimetric assay, by Fujifilm (1 mentions) Fgf21, by Merck Group (1 mentions)

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Luminex 200 (61 citations) Analyzer 3.1 Luminex 200 machine (3 citations)

8 protocols using luminex 200 machine

Measuring Metabolic Biomarkers in Plasma

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Blood glucose was measured with an Ascensia Breeze 2 glucometer (Bayer). Insulin, IL6, and leptin in plasma were measured by multiplexed ELISA (Millipore Sigma MMHMAG-44K) using a Luminex 200 machine (Millipore Sigma). The plasma concentration of FGF21 (Millipore Sigma EZRMFGF21-26K) and adiponectin (Millipore Sigma EZMADP-60K) was measured by ELISA.

Han M.S., Perry R.J., Camporez J.P., Scherer P.E., Shulman G.I., Gao G, & Davis R.J. (2021). A feed-forward regulatory loop in adipose tissue promotes signaling by the hepatokine FGF21. Genes & Development, 35(1-2), 133-146.

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Quantifying Cytokine Levels in COVID-19

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We measured cytokine levels in equal volumes of healthy adult and COVID-19 patient plasma using a multiplex bead array for interleukin-8 (IL-8) and IL-6 (MilliporeSigma), according to the manufacturer's instructions, and analyzed them on a Luminex 200 machine.

Middleton E.A., He X.Y., Denorme F., Campbell R.A., Ng D., Salvatore S.P., Mostyka M., Baxter-Stoltzfus A., Borczuk A.C., Loda M., Cody M.J., Manne B.K., Portier I., Harris E.S., Petrey A.C., Beswick E.J., Caulin A.F., Iovino A., Abegglen L.M., Weyrich A.S., Rondina M.T., Egeblad M., Schiffman J.D, & Yost C.C. (2020). Neutrophil extracellular traps contribute to immunothrombosis in COVID-19 acute respiratory distress syndrome. Blood, 136(10), 1169-1179.

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Metabolic Biomarker Measurement Protocol

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Blood glucose was measured with an Ascensia Breeze 2 glucometer (Bayer). FGF21 was measured using the Rat/Mouse Fibroblast Growth Factor 21 ELISA kit (EZRMFGF21-26K) (Millipore). Adipokines and insulin in plasma were measured by multiplexed ELISA using a Luminex 200 machine (Millipore).

Vernia S., Cavanagh-Kyros J., Barrett T., Tournier C, & Davis R.J. (2016). Fibroblast growth factor 21 mediates glycemic regulation by hepatic JNK. Cell reports, 14(10), 2273-2280.

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Metabolic Biomarkers Measurement

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Blood glucose was measured with an Ascensia Breeze 2 glucometer (Bayer). β-Hydroxybutyrate was measured by colorimetric assays (Wako). Insulin and TSH in plasma were measured by multiplexed ELISA using a Luminex 200 machine (Millipore). T3 and T4 (Calbiotech) and FGF21 (Millipore) in plasma were measured using ELISA kits. Serum triglyceride was measured using a kit (Sigma). Lipoprotein analysis was performed by the University of Cincinatti Mouse Metabolic Phenotyping Center.

Vernia S., Cavanagh-Kyros J., Garcia-Haro L., Sabio G., Barrett T., Jung D.Y., Kim J.K., Xu J., Shulha H.P., Garber M., Gao G, & Davis R.J. (2014). The PPARα - FGF21 hormone axis contributes to metabolic regulation by the hepatic JNK signaling pathway. Cell metabolism, 20(3), 512-525.

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Plasma Insulin Measurement in Mouse

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Whole blood was collected from the mouse recipients from their submandibular vein under anesthesia which was administered as described in 2.1 on week 15 following implantation after an overnight 16-hour fasting. The blood was placed in EDTA containing tubes and the plasma was collected after a 15-minute centrifugation at 2,000 rcf and 4 °C. For insulin measurements, ELISA kit (Crystal Chem) used with 40 μL per sample and the assay was read with a Luminex 200 machine (Millipore). For the data analysis, the measurement found to be below the detectable cut-off, was replaced with 0.09 ng/mL which is the lowest detectable value of 0.09 ng/mL by the kit.

Tsagkaraki E., Guilherme A., Nicoloro S.M., Kelly M., Lifshitz L.M., Wang H., Min K., Rowland L.A., Santos K.B., Wetoska N., Friedline R.H., Maitland S.A., Chen M., Weinstein L.S., Wolfe S.A., Kim J.K, & Czech M.P. (2023). Crosstalk between corepressor NRIP1 and cAMP signaling on adipocyte thermogenic programming. Molecular Metabolism, 76, 101780.

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Cytokine Profiling in Tumor Tissues

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Normal and tumor tissue pieces were divided into 8 mg (± 0.5 mg) sections and incubated in RPMI complete media for 16 h. Supernatants were analyzed by multiplex bead array for 26 cytokines/growth factors (Millipore, Billerica, MA) according to manufacturer’s instructions and analyzed on a Luminex 200 machine.

Qeadan F., Bansal P., Hanson J.A, & Beswick E.J. (2020). The MK2 pathway is linked to G-CSF, cytokine production and metastasis in gastric cancer: a novel intercorrelation analysis approach. Journal of Translational Medicine, 18, 137.

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Cytokine Profiling of Tumor Tissues

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8 mg (± 0.5 mg) of tumor tissues were incubated in complete RPMI media for 16 hours. Supernatants were analyzed by multiplex bead arrays (Millipore, Billerica, MA) for MK2 downstream cytokine levels (IL-1β, IL-6, and TNF-α), according to manufacturer’s instructions and analyzed on a Luminex 200 machine.

Ray A.L., Berggren K.L., Cruz S.R., Gan G.N, & Beswick E.J. (2017). Inhibition of MK2 suppresses IL-1β, IL-6, and TNF-α dependent colorectal cancer growth. International journal of cancer, 142(8), 1702-1711.

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Metabolic Biomarker Quantification

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Blood glucose was measured with an Ascensia Breeze 2 glucometer (Bayer, Pittsburgh, PA). Adipokines and insulin in plasma were measured by multiplexed ELISA using a Luminex 200 machine (Millipore, Billerica, MA).

Vernia S., Morel C., Madara J.C., Cavanagh-Kyros J., Barrett T., Chase K., Kennedy N.J., Jung D.Y., Kim J.K., Aronin N., Flavell R.A., Lowell B.B, & Davis R.J. (2016). Excitatory transmission onto AgRP neurons is regulated by cJun NH2-terminal kinase 3 in response to metabolic stress. eLife, 5, e10031.

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