Ucp 1

Oligonucleotide primers specific for glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SREBP-1c, C/EBPα, PPARγ, UCP-1, αSMA, and Col1α1 were purchased from Bioneer (Daejeon, Korea). M-MLV reverse transcriptase and random Oligonucleotide primers were from Promega (Fitchburg, WI, USA). TOP script RT Dry MIX was from Enzynomics Co., Ltd. (Daejeon, Korea). Thunderbird SYBR quantitative polymerase chain reaction (qPCR) Mix was from TOYOBO Co., Ltd. (Osaka, Japan). All other chemicals were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).

Dulbecco’s modified Eagle’s minimum essential medium (DMEM), fetal bovine serum (FBS), fetal calf serum (FCS), penicillin, and streptomycin were obtained from Gibco (Grand Island, NY). Monoclonal antibodies against ARG-1, TMEM26, PPARγ and β-actin were from Santa Cruz Biotechnology (Delaware, CA). Antibodies against UCP-1 and PPARδ were from Bioworld Technology (Minneapolis, MN). Peroxidase-conjugated secondary antibodies were from the Jackson Laboratory (Sacramento, CA), and enzyme immunoassay (EIA) kits for catecholamines were from R&D Systems (Minneapolis, MN). Random oligonucleotide primers and M-MLV reverse transcriptase were from Promega (Fitchburg, WI). TOPscriptTM RT Dry MIX was from Enzynomics (Daejeon, Korea). Thunderbird SYBR qPCR Mix was from Toyobo (Osaka, Japan). ARG-1, MRC-1, IL-10, UCP-1, Cited1, COX7a1, TMEM26 and GAPDH oligonucleotide primers were from Bioneer (Daejeon, Korea). Phenylmethylsulfonyl fluoride (PMSF), GW9662, GSK0660, telmisartan, triiodothyronine (T3) and all other chemicals were from Sigma Chemical (St. Louis, MO).