Rdrp reverse primer

Samples were homogenized in an Omni Bead ruptor in TRIzol, and then the RNA was extracted from 300 μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research); 2 μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first-strand cDNA synthesis kit (Thermo Fisher Scientific) in a 20-μL total reaction volume. For qPCR for SARS2 Rdrp, 20-μL reactions were prepared using 2 μL cDNA, 1 μL 10 mM Rdrp Forward primer (10006860; Integrated DNA Technologies), 1 μL Rdrp Reverse primer (10006881; Integrated DNA Technologies), and 10 μL 2x SYBR Green (Thermo Fisher Scientific). The reactions were then run on a 7500 Fast Dx Real-Time PCR Instrument (4357362R; Applied Biosystems). For qPCR for murine glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 20-μL reactions were prepared using 2 μL cDNA, 1 μL of a 20x murine GAPDH primer (MM.pt.39a.1; Integrated DNA Technologies), and 10 μL 2x SYBR Green. The reactions were then run on a QuantStudio 5 Real-Time PCR Instrument (A28133; Applied Biosystems).

Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher, Waltham, MA) in a 20μL volume. For qPCR for SARS2 Rdrp, 20μL reactions were prepared using 2μL cDNA, 1μL of 10mM Rdrp Forward primer (10006860, Integrated DNA Technologies, Coralville, IA), 1μL of Rdrp Reverse primer (10006881, Integrated DNA Technologies, Coralville, IA), and 10μL of 2x SYBR Green (Thermo Fisher, Waltham, MA). The reactions were then run on a 7500 Fast Dx Real-Time PCR Instrument (4357362R, Applied Biosystems, Waltham, MA). For qPCR for murine GAPDH, 20μL reactions were prepared using 2μL cDNA, 1μL of a 20x murine GAPDH primer (MM.pt.39a.1, Integrated DNA Technologies, Coralville, IA), and 10μL of 2x SYBR Green. The reactions were then run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA).