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Click it tunel alexa flour 594

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The Click-IT TUNEL Alexa Fluor 594 is a fluorescent labeling kit used to detect and quantify DNA fragmentation in apoptotic cells. It utilizes a copper-catalyzed azide-alkyne cycloaddition (click) reaction to incorporate a fluorescent dye into the DNA strand breaks, enabling visualization and analysis of apoptotic cells.

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Lab products found in correlation

Pezgs0416, by Merck Group (2 mentions)

2 protocols using click it tunel alexa flour 594

1

Quantifying Apoptosis in Retinal Cells

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To determine the percentage of apoptotic cells, retinal PC were plated at 1 × 104 cells per well of a four‐chamber slide (Cat#: PEZGS0416; Millipore). The next day, medium was replaced with medium containing 10 μmol/L 1,25(OH)2D3 or solvent ethanol‐treated, and cells were incubated for 48 hours. TdT‐dUTP terminal nick‐end labeling (TUNEL) staining was performed using Click‐IT TUNEL Alexa Flour 594, as recommended by the supplier (Cat#: C10246; Life Technologies). Positive cells were counted per five high power field (×200) using a fluorescence microscope and reported as a percentage of apoptotic cells relative to total number of cells per field. DNase I treatment was used as a positive control as recommended by the supplier.
Jamali N., Song Y., Sorenson C.M, & Sheibani N. (2019). 1,25(OH)2D3 regulates the proangiogenic activity of pericyte through VDR‐mediated modulation of VEGF production and signaling of VEGF and PDGF receptors. FASEB bioAdvances, 1(7), 415-434.
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2

Quantifying Retinal Cell Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the percentage of apoptotic cells, retinal PC were plated at 1 × 104 cells per well of a four-chamber slide (Cat#: PEZGS0416; Millipore). The next day, medium was replaced with medium containing 10 μmol/L 1,25(OH)2D3 or solvent ethanol-treated, and cells were incubated for 48 hours. TdT-dUTP terminal nick-end labeling (TUNEL) staining was performed using Click-IT TUNEL Alexa Flour 594, as recommended by the supplier (Cat#: C10246; Life Technologies). Positive cells were counted per five high power field (×200) using a fluorescence microscope and reported as a percentage of apoptotic cells relative to total number of cells per field. DNase I treatment was used as a positive control as recommended by the supplier.
Jamali N., Song Y., Sorenson C.M, & Sheibani N. (2019). 1,25(OH)2D3 regulates the proangiogenic activity of pericyte through VDR‐mediated modulation of VEGF production and signaling of VEGF and PDGF receptors. FASEB bioAdvances, 1(7), 415-434.
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