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2 protocols using udp glucose

1

Biochemical Compound Acquisition Protocol

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4-Hydroxy-2,5-dimethylfuran-3(2H)-one (HDMF), 2 (or 5)-ethyl-4-hydroxy-5 (or 2)-methylfuran-3(2H)-one (EHMF), 4-hydroxy-5-methylfuran-3(2H)-one (HMF), naringenin, eugenol, vanillic acid, salicylic acid, 1-naphthol, farnesol, sorbic acid, nerolidol, geraniol, fisetin, vanillin, chlorogenic acid, 2-naphthol, caffeic acid, methyl salicylate, ferulic acid, 1-octanol, and myricetin were purchased from Sigma-Aldrich (Shanghai, China). UDP-glucose, UDP-galactose, and UDP-glucuronic acid were purchased from Promega (Madison, USA). UDP-Xylose was purchased from Angfei Biotech Co., Ltd. (Guangzhou, China).
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2

Purification and Assay of TreT Enzyme

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TreT was expressed and purified from E. coli as previously described.[52 ] The UDP-Glo assay kit, including ultra-pure UDP-glucose, were obtained from Promega. 18F-2-FDG was purchased from IBA Molecular (Madrid, Spain) or PetNet Solutions (Culver City, CA). Non-radioactive 19F-2-FDG was purchased from CarboSynth. UDP-glucose was obtained from Sigma or Abcam. AG1-X8 mixed-bed ion exchange resin and Poly-Prep columns were purchased from Bio-Rad Laboratories.
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