HRW was prepared by using a hydrogen water generator from Hydrogen (Osmo-star Soriano S.L., Alicante, Spain), that uses electrolysis method to break down H2O into H2. HRW was intraperitoneally injected 1 h before testing at a concentration of 0.3 mM. Tin protoporphyrin IX (SnPP) and CoPP were acquired from Frontier Scientific (Livchem GmbH & Co., Frankfurt, Germany), and dicoumarol was purchased from Eurodiagnostico S.L. (Madrid, Spain). All of them were dissolved in dimethyl sulfoxide (DMSO, 1% solution in saline (0.4% NaCl). Glibenclamide purchased from Sigma–Aldrich (St. Louis, MO, USA) was dissolved in saline solution. SnPP, Glibenclamide and dicoumarol were administered 30 min before testing at a dose of 10 mg/kg, and CoPP at 3 h prior to the tests at dose of 2.5 mg/kg according to earliest studies [13 (link),31 (link)]. All drugs were prepared right before their use and were intraperitoneally injected in a final volume of 10 mL/kg. For each treatment, the respective control group received the corresponding vehicle (VEH).
Tin protoporphyrin 9
Manufactured by Frontier Scientific
Sourced in Germany, United States
Tin protoporphyrin IX is a chemical compound used in laboratory settings. It functions as a metalloporphyrin, a class of compounds that contain a central metal ion coordinated within a porphyrin ring structure.
Automatically generated - may contain errors
Lab products found in correlation
Glibenclamide, by Merck Group (1 mentions)
4 morpholineethanesulfonic acid, by Merck Group (1 mentions)
Poloxamer 407, by Merck Group (1 mentions)
Tween 80, by Thermo Fisher Scientific (1 mentions)
Gibco dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
Hematoxylin and eosin h e stain, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
Glycerol, by Mallinckrodt (1 mentions)
2 protocols using tin protoporphyrin 9
1
Hydrogen-Rich Water and Heme Oxygenase Modulators
2
Optimized Staining and Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tin protoporphyrin IX, deuteroporphyrinIX-2,4-disulfonic acid, and 10% neutral buffered
formalin were purchased from Frontier Scientific (Logan, UT, USA) and RICCA Chemical
Company (Arlington, TX, USA), respectively. Gibco Dulbecco’s Phosphate-Buffered
Saline (DPBS) was obtained from Life Technologies Corporation (Grand Island, NY, USA).
Phosphate buffered saline (PBS), 45% sodium polyacrylate solution, poloxamer 407,
hematoxylin and eosin (H&E) stain, acetonitrile, dimethylformamide,
4-morpholineethanesulfonic acid, methanol, sodium hydroxide, hydrochloric acid, 7H9 and
7H11 media were obtained from Sigma–Aldrich Chemical Co. (St Louis, MO, USA).
Glycerol and oleic acid-albumin-dextrose-catalase (OADC) were procured from Mallinckrodt
Pharmaceuticals (Staines-Upon-Thames, Surrey, U.K.) and BD Biosciences (Billerica, MA,
USA), respectively. Tween 80 was purchased from Thermo Fisher Scientific (Washington, DC,
USA).
formalin were purchased from Frontier Scientific (Logan, UT, USA) and RICCA Chemical
Company (Arlington, TX, USA), respectively. Gibco Dulbecco’s Phosphate-Buffered
Saline (DPBS) was obtained from Life Technologies Corporation (Grand Island, NY, USA).
Phosphate buffered saline (PBS), 45% sodium polyacrylate solution, poloxamer 407,
hematoxylin and eosin (H&E) stain, acetonitrile, dimethylformamide,
4-morpholineethanesulfonic acid, methanol, sodium hydroxide, hydrochloric acid, 7H9 and
7H11 media were obtained from Sigma–Aldrich Chemical Co. (St Louis, MO, USA).
Glycerol and oleic acid-albumin-dextrose-catalase (OADC) were procured from Mallinckrodt
Pharmaceuticals (Staines-Upon-Thames, Surrey, U.K.) and BD Biosciences (Billerica, MA,
USA), respectively. Tween 80 was purchased from Thermo Fisher Scientific (Washington, DC,
USA).
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