Human dental pulp stem cells (DPSC) were obtained from Lonza (PT-5025). The stemness of human dental pulp stem cells (DPSCs) (p. 16) was confirmed by flow cytometry as previously described [30 (link)]. The expression of positive (CD105) and negative (CD33) markers of mesenchymal stem cells was analyzed, using a BD FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA). Briefly, cells were stained with PE anti-human CD105 antibody (323205, Biolegend, San Diego, CA, USA) and APC anti-human CD33 antibody (303407, Biolegend, San Diego, CA, USA), following the manufacturer’s instructions.
Pe anti human cd105 antibody
Manufactured by BioLegend
Sourced in United States
PE anti-human CD105 antibody is a fluorochrome-conjugated monoclonal antibody that specifically binds to the human CD105 antigen. CD105, also known as endoglin, is a type I membrane glycoprotein that is expressed on endothelial cells and is involved in angiogenesis and cell proliferation. This antibody can be used for the identification and enumeration of CD105-positive cells in flow cytometry applications.
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2 protocols using pe anti human cd105 antibody
1
Characterization of Dental Pulp Stem Cells
2
Flow Cytometry Analysis of MSC Markers
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To determine the expression of MSC surface antigens, infrapatellar fat pad-MSCs at Passage 3 were analyzed by flow cytometry. Briefly, cells were lifted using 0.25% trypsin (Gibco). Cells were stained with dye-conjugated antibody, including fluorescein antihuman CD34 antibody, PE antihuman CD45 antibody, fluorescein antihuman CD44 antibody, APC/Cyanine 7 (Cy7) antihuman CD73 antibody, PE/Cy7 antihuman CD90 antibody, and PE antihuman CD105 antibody (Biolegend). The stained cells were analyzed with flow cytometry (Beckman Coulter DXFLEX).
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