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E cadherin af748

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E-cadherin (AF748) is a laboratory reagent produced by R&D Systems. It functions as a cell-cell adhesion molecule that plays a role in the maintenance of tissue architecture and cell polarity.

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Lab products found in correlation

Ab3517, by Abcam (1 mentions) β actin sc 1616, by Santa Cruz Biotechnology (1 mentions) Cellytic mt cell lysis reagent, by Merck Group (1 mentions) Imagequant las3000, by Fujifilm (1 mentions) Ab9570, by Abcam (1 mentions) Ab194726, by Abcam (1 mentions) Ab241332, by Abcam (1 mentions) Gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions) β catenin c2206, by Merck Group (1 mentions) Sc 8396, by Santa Cruz Biotechnology (1 mentions) P β catenin sc 16743 r, by Santa Cruz Biotechnology (1 mentions) Sc 21731, by Santa Cruz Biotechnology (1 mentions) Ab16502, by Abcam (1 mentions) Ab15160, by Abcam (1 mentions) Ab15580, by Abcam (1 mentions) Muc2 sc 15334, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

AF748 E-cadherin

3 protocols using e cadherin af748

1

Western Blot Analysis of Cell Lysates

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Protein samples were prepared by CelLytic MT Cell Lysis Reagent (Sigma-Aldrich St. Louis, MO) supplemented with protease inhibitor cocktail, which contained 2 µg/mL of aprotinin, 2 µg/mL of leupeptin, and 100 µmol/L of phenylmethylsulfonyl fluoride. Then, 20 mg of the protein lysate was resolved by SDS–PAGE on 8% or 10% gels, transferred to polyvinylidene difluoride membranes, and probed with antibodies against Clock (ab3517; Abcam, Cambridge, UK), E-cadherin (AF748; R and D systems, Minneapolis, MN), Claudin1 (13050–1-AP; proteintech, Rosemont, IL), Vimentin (MAB21052; R and D systems), p84 (THOC1, 10920–1-AP; proteintech), and β-Actin (sc-1616; SantaCruz Biotechnology, Texas, TX). Specific antigen–antibody complexes were visualized using horseradish peroxidase–conjugated secondary antibodies and a chemiluminescence reagent. The membranes were photographed, and the density of each band was analyzed with an ImageQuant LAS 3000 (Fuji Film, Japan).
Ogino T., Matsunaga N., Tanaka T., Tanihara T., Terajima H., Yoshitane H., Fukada Y., Tsuruta A., Koyanagi S, & Ohdo S. (2021). Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells. eLife, 10, e66155.
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2

Comprehensive Protein Expression Analysis

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Western blot analysis was carried out on whole cell lysates. Antibodies against Tim-3 (Ab241332), NF-κB (p65) (Ab16502), p-NF-κB (p-p65) (Ab194726) and vascular endothelial growth factor (VEGF) A (Ab9570) were purchased from (Abcam Cambridge, UK). GAPDH (sc-47724), p-β-catenin (sc-16743-R), cyclin D1 (CCND1) (sc-8396), C-Myc (sc-70465), matrix metalloproteinase-1 (MMP1) (sc-21731), TWIST (sc-6269), zona occludens (ZO)-1 (sc-10804), ZO-2 (sc-11448), occludin (sc-133256) and VEGFB (sc-13083) antibodies were obtained from Santa Cruz (Insight Biotechnology Limited, Middlesex UK). E-Cadherin (AF748) and VEGFD (MAB286) antibodies were purchased from R&D Systems (Abingdon, Oxfordshire, UK). STAT3 (S5933), p-STAT3 (SAB4504541), interleukin (IL)-6 (17901) and β-catenin (C2206) antibodies were purchased from Sigma-Aldrich (Gillingham, Dorset, UK). Anti-mouse (A5278), anti-rabbit (A0545) and anti-goat (A8919) secondary antibodies were obtained from Sigma-Aldrich (Gillingham, Dorset, UK).
Cong Y., Cui Y., Zhu S., Cao J., Zou H., Martin T.A., Qiao G., Jiang W, & Yu Z. (2020). Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells. Chinese Journal of Cancer Research, 32(5), 564-579.
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3

Intestinal Crypt Enteroid Culture and Cytokine Treatment

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Primary intestinal crypt cultures (enteroids) were established and maintained in culture according to the methods published by Sato et al. (11 (link)) with a few modifications (9 (link), 12 (link)). Enteroids were seeded on Matrigel, allowed to grow for at least 24 h before treatment with recombinant rat IL-17A (Peprotech – 100 ng/mL) or vehicle alone for 6 hours in order to evaluate cell proliferation, differentiation and death by immunohistochemistry (IHC) and confocal microscopy as described in the next section. The following antibodies were used for IHC analysis: BrdU (BRD494 – Novus Biosciences), chromogranin A (ab15160 – Abcam), e-cadherin (AF748 – R&D Systems), Ki67 (ab15580 – Abcam) and mucin glycoprotein 2 (Muc2, sc-15334 – Santa Cruz). Nuclear counterstaining was performed using DAPI (Pierce).
All-trans retinoic acid (ATRA) was obtained from Sigma-Aldrich, dissolved in DMSO and corn oil (1:1 – final concentration 6 mg/mL, protected from light) and administered daily by gavage to breast-fed and NEC mice (50 μg/mouse) for the duration of the experimental induction of NEC.
Niño D.F., Sodhi C.P., Egan C.E., Zhou Q., Lin J., Lu P., Yamaguchi Y., Jia H., Martin L.Y., Good M., Fulton W.B., Prindle T J.r., Ozolek J.A, & Hackam D.J. (2017). Retinoic Acid Improves Incidence and Severity of Necrotizing Enterocolitis by Lymphocyte Balance Restitution and Repopulation of Lgr5+ Intestinal Stem Cells. Shock (Augusta, Ga.), 47(1), 22-32.
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