Expression analysis of selected genes was performed by reverse transcription quantitative PCR (RT-qPCR) using the primers listed in Supplementary Table S2 . RNA was extracted using the NucleoSpin(R) RNA Plant Kit (Machery-Nagel, Düren, Germany). RNA transcription into cDNA was performed using the qScript cDNA Synthesis Kit (Quantbio, Beverly, United States). Expression was analyzed in three biological replicates for each tested gene under each test condition. Relative expression was calculated via the ΔCT method relative to the expression of BvUGD1 (BVRB_7g172340; Supplementary Table S2 ). Detailed information on the primers used for quantification is listed in Supplementary Table S2 .
Nucleospin r rna plant kit
Manufactured by Macherey-Nagel
Sourced in Germany
The NucleoSpin® RNA Plant Kit is a lab equipment product designed for the isolation of high-quality total RNA from plant tissues. It utilizes a silica-membrane technology to efficiently capture and purify RNA molecules from various plant sources.
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Lab products found in correlation
2 protocols using nucleospin r rna plant kit
1
Gene Expression Analysis by RT-qPCR
2
RT-qPCR Analysis of Sugar Beet Genes
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Expression analysis of selected genes was performed by reverse transcription quantitative PCR (RT-qPCR) using the primers listed in Supplemental Table S2. RNA was extracted from homogenized sugar beet tissue using the NucleoSpin(R) RNA Plant Kit (Machery-Nagel, Düren, Germany) according to the manufacturer's guidelines. RNA transcription into cDNA was performed using the qScript cDNA Synthesis Kit (Quantbio, Beverly, USA).
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