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Anti pe and anti apc magnetic beads

Manufactured by Miltenyi Biotec
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Anti-PE and anti-APC magnetic beads are laboratory reagents used for cell separation and enrichment applications. The beads are coated with antibodies that specifically bind to the PE (Phycoerythrin) or APC (Allophycocyanin) fluorescent labels, allowing for the isolation of cells or molecules labeled with these fluorescent markers.

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Lab products found in correlation

Anti cd8 bv605, by BioLegend (2 mentions) Sytox green viability dye, by Thermo Fisher Scientific (2 mentions) Bv421 klrg1, by BioLegend (2 mentions) Pe cy7 pd 1, by BioLegend (2 mentions) Cd45ra af700, by BD (2 mentions) Ccr7 apc cy7, by BioLegend (2 mentions) Percp efluor 710 tigit, by Thermo Fisher Scientific (2 mentions) Bv650 cd95, by BioLegend (2 mentions) Anti cd4 cd14 cd16 cd20 cd40 fitc dump, by Thermo Fisher Scientific (2 mentions) Allprep dna rna mini prep kit, by Qiagen (2 mentions) Facsaria 2, by BD (2 mentions) Anti cxcr5 bv421, by BioLegend (1 mentions) Zombie aqua viability dye, by BioLegend (1 mentions) Anti cd14 bv510 clone m5e2, by BioLegend (1 mentions) Clone j252d4, by BioLegend (1 mentions) Lsrfortessa x 20, by BD (1 mentions)

3 protocols using anti pe and anti apc magnetic beads

1

SARS-CoV-2 Antigen-Specific T Cell Profiling

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Cryopreserved samples containing 1-10 × 107 PBMC from DRB1*07:01 (DR7)+ convalescent subjects or pre-pandemic negative controls were thawed, washed twice with PBS containing 2% FBS, and resuspended in either complete EHAA or RPMI 1640 with 5% FBS. Staining with a pre-mixed cocktail of DR7:p-streptavidin-PE, DR7:p-streptavidin-PE-Cy7, and DR7:p-streptavidin-APC tetramers at a final concentration of 10 nM for each reagent was for 1 hour at room temperature. Anti-PE and anti-APC magnetic beads (Miltenyi Biotec) were then added, and bead-bound cells were enriched as previously described (22). Cells in the bound and unbound fractions were stained with Zombie Aqua viability dye (BioLegend) and a surface marker antibody panel consisting of anti-CD20BV510 (clone 2H7, BioLegend), anti-CD14BV510 (clone M5E2, BioLegend), anti-CD3AF700 (clone UCHT1, BioLegend), anti-CD4BV605 (clone OKT4, BioLegend), anti-CD8BUV395 (clone RPA-T8, BioLegend), anti-CD45ROAPC/Cy7 (clone UCHL1, BioLegend), anti-CXCR3PE/Dazzle594 (clone G025H7, BioLegend), anti-CXCR5BV421 (clone J252D4, BioLegend), anti-PD-1BV785 (clone EH12.2H7, BioLegend), and anti-CCR4PerCP/Cy5.5 (clone L291H4, BioLegend) for 30 min at room temperature. Data were acquired on an LSR Fortessa X-20 (BD) flow cytometer and analyzed with FlowJo software (BD).
Nelson R.W., Chen Y., Venezia O.L., Majerus R.M., Shin D.S., Carrington M.N., Yu X.G., Wesemann D.R., Moon J.J, & Luster A.D. (2022). SARS-CoV-2 epitope-specific CD4+ memory T cell responses across COVID-19 disease severity and antibody durability. Science Immunology.
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2

Multiparametric Flow Cytometry Profiling of Antigen-specific CD8+ T Cells

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Samples from HLA-A2–positive subjects were dual stained with a mixture containing a pool of PE and APC tetramers, PBMCs (20 ×10^6) were stained simultaneously with 3 μl each in 200 μl of PBS supplemented with 2% BSA and incubated for 15 min at 37°C. Cells were then incubated with 30 μl each of anti-PE and anti-APC magnetic beads (Miltenyi Biotec, San Diego, CA, USA) and enriched on a MS-sized magnetic column according to the manufacturer’s instructions. After enrichment, flow-through cells were reserved for bulk memory and naive CD8+ T-cell sorting described in the following section. Both enriched and flowthrough cells samples were washed and then stained for 30 min at 4°C with 5 μl each:anti- CD8+ BV605(Biolegend), AF700 CD45RA(BD Bioscience), APC-Cy7 CCR7(Biolegend), PerCP- eFluor 710 TIGIT(eBioscience), PE-Cy7 PD-1(Biolegend), BV650 CD95(Biolegend), BV421 KLRG1(Biolegend), 2.5 μl each of anti-CD4/CD14/CD16/CD20/CD40-FITC (dump channel; eBioscience, Waltham, MA, USA), and 1:10,000 syTOX green viability dye(Thermo Fisher). CD8+ cells were sorted using a FACSAria II(BD Biosciences) into lysis buffer from an Allprep DNA RNA mini prep kit (Qiagen) and processed for DNA and RNA according to the manufacturer’s instructions.
Abdelsamed H.A., Zebley C.C., Nguyen H., Rutishauser R.L., Fan Y., Ghoneim H.E., Crawford J.C., Alfei F., Alli S., Ribeiro S.P., Castellaw A.H., McGargill M.A., Jin H., Boi S.K., Speake C., Serti E., Turka L.A., Busch M.E., Stone M., Deeks S.G., Sekaly R.P., Zehn D., James E.A., Nepom G.T, & Youngblood B. (2020). Beta cell-specific CD8+ T cells maintain stem-cell memory-associated epigenetic programs during type 1 diabetes. Nature immunology, 21(5), 578-587.
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3

Multiparametric Flow Cytometry Profiling of Antigen-specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples from HLA-A2–positive subjects were dual stained with a mixture containing a pool of PE and APC tetramers, PBMCs (20 ×10^6) were stained simultaneously with 3 μl each in 200 μl of PBS supplemented with 2% BSA and incubated for 15 min at 37°C. Cells were then incubated with 30 μl each of anti-PE and anti-APC magnetic beads (Miltenyi Biotec, San Diego, CA, USA) and enriched on a MS-sized magnetic column according to the manufacturer’s instructions. After enrichment, flow-through cells were reserved for bulk memory and naive CD8+ T-cell sorting described in the following section. Both enriched and flowthrough cells samples were washed and then stained for 30 min at 4°C with 5 μl each:anti- CD8+ BV605(Biolegend), AF700 CD45RA(BD Bioscience), APC-Cy7 CCR7(Biolegend), PerCP- eFluor 710 TIGIT(eBioscience), PE-Cy7 PD-1(Biolegend), BV650 CD95(Biolegend), BV421 KLRG1(Biolegend), 2.5 μl each of anti-CD4/CD14/CD16/CD20/CD40-FITC (dump channel; eBioscience, Waltham, MA, USA), and 1:10,000 syTOX green viability dye(Thermo Fisher). CD8+ cells were sorted using a FACSAria II(BD Biosciences) into lysis buffer from an Allprep DNA RNA mini prep kit (Qiagen) and processed for DNA and RNA according to the manufacturer’s instructions.
Abdelsamed H.A., Zebley C.C., Nguyen H., Rutishauser R.L., Fan Y., Ghoneim H.E., Crawford J.C., Alfei F., Alli S., Ribeiro S.P., Castellaw A.H., McGargill M.A., Jin H., Boi S.K., Speake C., Serti E., Turka L.A., Busch M.E., Stone M., Deeks S.G., Sekaly R.P., Zehn D., James E.A., Nepom G.T, & Youngblood B. (2020). Beta cell-specific CD8+ T cells maintain stem-cell memory-associated epigenetic programs during type 1 diabetes. Nature immunology, 21(5), 578-587.
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