Inc 280

Four NSCLC cell lines were selected (Table 1), ranging in sensitivity to Erlotinib therapy. The cell lines HCC827, H1666, and H358 were acquired from American Type Culture Collection (Manassas, VA). PC-9 cells were kindly provided by Reen Wu (University of California, Davis, CA). All cell lines were maintained in RPMI 1640 supplemented with 10% fetal bovine serum (JR Scientific, Woodland, CA), penicillin/streptomycin/L-glutamine, and minimum essential medium vitamin solution (Invitrogen, Carlsbad, CA), as previously published.^{23 (link)} Cell line authentication for HCC827, PC-9, H1666, and H358 was performed by the University of Arizona Genetics Core on 2/3/14 comparing the autosomal STR profiles with reference databases. Erlotinib and INC-280 were purchased from Selleck Chemicals (Houston, TX). Both agents were diluted in dimethyl sulfoxide to a concentration of 10 mM. HGF was purchased from Peprotech (Rocky Hill, NJ) and reconstituted in 0.1% bovine serum albumin to a stock concentration of 10 μg/mL. Agents were stored at −20°C until use.