Ketamin
Manufactured by Pfizer
Sourced in Germany
Ketamin is a laboratory equipment product that functions as an anesthetic. It is used to induce anesthesia and provide pain relief in clinical and research settings. The core function of Ketamin is to act as a general anesthetic, suppressing the central nervous system and producing a dissociative state.
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Lab products found in correlation
Pazopanib, by Novartis (1 mentions)
Rumpon, by Bayer (1 mentions)
C57bl 6j mice, by Janvier Labs (1 mentions)
Ketavet, by Pfizer (1 mentions)
Papain, by Merck Group (1 mentions)
Dnase, by Merck Group (1 mentions)
Xylacin, by Bayer (1 mentions)
Rnalater, by Thermo Fisher Scientific (1 mentions)
Dnmt activity inhibition assay, by Active Motif (1 mentions)
Nuclear extract kit, by Active Motif (1 mentions)
5 protocols using ketamin
1
Pazopanib, Ketamin, and Taxifolin Study
2
Mouse Brain Tissue Fixation
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This study was carried out in strict accordance with national health and ethical regulations, and the care of animals was in accordance with institutional guidelines. The protocol was approved by the Committee on the Ethics of Animal Experiments of Freiburg University. Mice were studied at embryonic (E16), postnatal (P0-P15), and adult stages (3 months old). Pregnant mice were killed by cervical translocation, embryos were collected, and brains were immediately dissected and immersion-fixed in a 4 % paraformaldehyde (PFA) solution. Mice at postnatal ages were anesthetized by using 10 % ketamin (20 mg/kg, Pfizer) and 2 % Rumpon (4 mg/kg, Bayer Healthcare) and transcardially perfused with a freshly prepared 4 % PFA solution in phosphate-buffered saline (PBS, pH 7.2; Merck, Germany). Following perfusion, the brains were dissected and postfixed in 4 % PFA for at least 24 h.
3
Genetic Manipulation of Phospholipase D in Mice
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Animal studies were performed in accordance with the guidelines of the European Parliament for the use of living animals in scientific studies and in accordance with the German law for protection of animals. The protocol was approved by Heinrich-Heine-University Animal Care Committee and by the district government of North-Rhine-Westphalia (LANUV, NRW, AZ 84-02.04.2013.A486).
Gene-targeted mice lacking either PLD1 or PLD2 were described before (Dall’Armi et al., 2010 (link)). Both mutant mouse lines were intercrossed to create constitutive Pld1-/-/Pld2-/- mice and the corresponding wild-type littermates were bred from breeder pairs. For the analysis of the impact of PLD enzymatic activity on myocardial ischemia and reperfusion injury, treatment of C57BL/6J mice (Janvier Labs) with the PLD inhibitor FIPI were performed. Experiments were done with male mice aged 10–12 weeks. Mice were anesthetized with Ketamin (100 mg/kg Ketavet®, Pfizer, berlin, Germany) and Xylacin (5 mg/kg, Xylazin 2% Bernburg, Medistar, Ascheberg, Germany) by intraperitoneal (i.p.) injection before opening the thorax for removal of the heart or euthanasia was performed by cervical dislocation.
Gene-targeted mice lacking either PLD1 or PLD2 were described before (Dall’Armi et al., 2010 (link)). Both mutant mouse lines were intercrossed to create constitutive Pld1-/-/Pld2-/- mice and the corresponding wild-type littermates were bred from breeder pairs. For the analysis of the impact of PLD enzymatic activity on myocardial ischemia and reperfusion injury, treatment of C57BL/6J mice (Janvier Labs) with the PLD inhibitor FIPI were performed. Experiments were done with male mice aged 10–12 weeks. Mice were anesthetized with Ketamin (100 mg/kg Ketavet®, Pfizer, berlin, Germany) and Xylacin (5 mg/kg, Xylazin 2% Bernburg, Medistar, Ascheberg, Germany) by intraperitoneal (i.p.) injection before opening the thorax for removal of the heart or euthanasia was performed by cervical dislocation.
4
Neuronal Culture Protocol from Mice
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Cortex and hippocampi were removed from male adult (6–8 week old) mice and neurons were cultured using a protocol adapted from.68 (link) Briefly, mice were anesthetized with an I.P. injection of ketamin (Pfizer) and sacrificed by cervical transection. Hippocampi and cortex were surgically removed in dissection medium (Hibernate A, 1% gentamicin), cut into small pieces and digested with papain (Sigma, 12.5 U/mL, 45 min) at 30°C. papain was removed after incubation and and tissue pieces were triturated in 10% FBS and DNase (Sigma, 50 μg/mL) containing disection medium. Cells were pelleted by centrifugation at 180 g for 5 min at 4°C and resuspended in culture medium 10% horse serum containing NBA-B27. Cells were plated on poly-D-lysine coated glass bottom culture dishes and incubated in NBA-B27 at 37°C with 5% CO2 and 9% O2.
5
Epigenetic Profiling of Poly I:C-Exposed Mice
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At an age of thirteen weeks, brains from 8 male and 9 female mice prenatally exposed to Poly I:C, and 9 male and 9 female offspring from PBS-treated dams were used for epigenetic analysis. For this purpose, mice were anesthetized with a single intraperitoneal injection of Ketamin (Pfizer, Germany) and Xylacin (Bayer, Germany). 0.7 ml 10 % Ketamin and 0.3 ml 2 % Xylacin were mixed and administered at a dose of 10 ml/kg. Afterwards, brains were collected and stored at −20 °C in RNA-Later (Invitrogen, Germany). Half of the brain was used to extract nuclear proteins using a Nuclear Extract Kit (Active Motif, Belgium) and the activities of various HDAC and DNMT enzymes were analyzed with the HDAC Fluorescent Assay Kit and DNMT Activity/Inhibition Assay (Active Motif, Belgium). The fluorescence/optical density was detected with a PolarStar OPTIMA microplate fluorimeter (BMG Labtech).
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