Prodigy ods3 column

Manufactured by Phenomenex

Sourced in Japan, United States

The Prodigy ODS3 column is a reversed-phase liquid chromatography column designed for the separation and analysis of a wide range of organic compounds. It features a 3 μm particle size and a C18 stationary phase, providing efficient and high-resolution separations.

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Lab products found in correlation

Lc 20ad hplc, by Shimadzu (2 mentions) Chromatography system, by Phenomenex (1 mentions) Sil 20a autosampler, by Shimadzu (1 mentions) 515 hplc pumps, by Thermo Fisher Scientific (1 mentions)

5 protocols using prodigy ods3 column

Peptide Characterization by Mass Spectrometry

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The peptides
were characterized
by mass spectrometry on a Bruker Daltonics UltrafleXtreme matrix-assisted
desorption ionization time-of-flight mass spectrometer operating in
positive-ion reflector mode [matrix of α-cyano-4-hydroxycinnamic
acid (CHCA), external calibration]. High-resolution mass spectrometry
was performed on a Waters Synapt G2-S nano-ESI-IMS-TOF mass spectrometer.
Analytical HPLC measurements were performed using a JASCO chromatography
system and a Phenomenex Prodigy ODS-3 column (5 μm, 4.6 mm ×
100 mm). For peptide characterization, a linear gradient of water
and acetonitrile (buffer A consisted of water and 0.1% TFA, and buffer
B consisted of acetonitrile and 0.1% TFA) run from 20 to 80% over
20 min was used. Chromatograms were monitored at wavelengths of 220
and 280 nm.

Thomas F., Niitsu A., Oregioni A., Bartlett G.J, & Woolfson D.N. (2017). Conformational Dynamics of Asparagine at Coiled-Coil Interfaces. Biochemistry, 56(50), 6544-6554.

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Quantitative HPLC Analysis of Ergot Alkaloids

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Ergot alkaloids were analyzed by high performance liquid chromatography (HPLC) with fluorescence detection as described by
Panaccione et al. (2012) (link). Briefly, samples were separated on a Prodigy ODS3 column
(5-μm particle size; 150 mm by 4.6 mm; Phenomenex, Torrance, CA) with a multilinear gradient from 5% acetonitrile + 95% 50
mM ammonium acetate to 75% acetonitrile + 25% 50 mM ammonium acetate. Analytes were detected in two serially arranged fluorescence
detectors; one with excitation and emission wavelengths of 310 nm and 410 nm, respectively, to detect lysergic acid derivatives,
and the other at 272 nm/372 nm to detect ergot alkaloids lacking the lysergic acid fluorophore.

Cook D., Lee S.T., Panaccione D.G., Leadmon C.E., Clay K, & Gardner D.R. (2019). Biodiversity of Convolvulaceous species that contain Ergot Alkaloids, Indole Diterpene Alkaloids, and Swainsonine. Biochemical systematics and ecology, 86, 103921.

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Phenolic Analysis of Olive Mill Wastewater

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The analysis of the phenolic component in OMWP was carried out as previously described with some modifications (Navarro, Fiore, Fogliano & Morales, 2015) . Briefly, the OMWP was dissolved in distilled water, in order to prepare a solution at a concentration of 20 mg/mL, spiked with 10 μL of a 5 mg/L solution of butyl-4-hydroxybenzoate as the internal standard. The phenolic fraction was purified through polymeric Strata X C18 cartridges (Phenomenex, Torrance, CA), previously activated with methanol and water. Cartridges were further washed with 3 mL of water, and 1 mL of methanol was collected and dried under a gentle nitrogen stream. The purified fraction was dissolved in 500 μL of a solution of 5% methanol in water. An LC-20AD HPLC with a UV-Vis detector SPD20A, set at 279 nm, and a SIL-20A autosampler (Shimadzu, Kyoto, Japan) was used, while chromatographic separation was achieved through a Prodigy ODS3 column (250 × 4.60 mm, 5 μm, Phenomenex). Mobile phases consisted of water (A) and methanol (B) and the following gradient was used: 0 min (5% B); 4 min (5% B); 40 min (98% B); 43 min (98% B).
Hydroxytyrosol, tyrosol and verbascoside were quantified by external calibration technique according to the procedure detailed by Navarro and coworkers (Navarro, Fiore, Fogliano & Morales, 2015) .

Troise A.D., Colantuono A, & Fiore A. (2020). Spray-dried olive mill wastewater reduces Maillard reaction in cookies model system. Food chemistry, 323.

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HPLC Analysis of Radiolabeled Compounds

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The HPLC instrumentation included two Waters (Milford, MA) 515 HPLC pumps, an Applied BioSystems (Foster City, CA) 759A absorbance detector, and a β-RAM Model 3 radioactivity detector with a 50-μL lithium glass solid scintillant cell (IN/US, Tampa, FL). A Phenomenex (Torrance, CA) Prodigy ODS3 column (250 × 4.6 mm, 5 μm) was used with mobile phase A: 75% aqueous acetonitrile and B: acetonitrile and a flow rate of 1 ml/min. The initial mobile phase composition of 100% A was held for 12 min before changing linearly to 100% B over 3 min, and then held at 100% B for 5 min before returning to initial conditions. The eluent monitored for both UV absorbance at 220 and 254 nm and radioactivity.

Waidyanatha S., Black S.R., Patel P.R., Watson S.L., Snyder R.W., Sutherland V., Stanko J, & Fennell T.R. (2020). Disposition and metabolism of antibacterial agent, triclocarban, in rodents; a species and route comparison. Xenobiotica; the fate of foreign compounds in biological systems, 50(12), 1469-1482.

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HPLC Analysis of Olive Mill Wastewater Phenolics

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The analysis of the phenolic component was carried out as described by Kokkinidou & Peterson16 with some modifications. Briefly, the OMW powder was dissolved in distilled water, in order to prepare a solution at a concentration of 20 mg mL-1. To 1 mL of this solution, 10 μL of a 5 mg L-1 solution of butyl-4-hydroxybenzoate as internal standard were added. The phenolic fraction was extracted through the use of SPE cartridges Strata C18-E, and dried under a nitrogen stream. Thereafter the precipitate was recovered in 500 μL of a solution of water-methanol/95 : 5 (v/v) ready for HPLC analysis. The instrument used for chromatographic analysis was an LC-20AD HPLC with a UV-Vis detector SPD20A, set at 279 nm, and an SCL-20A controller (Shimadzu, Japan). The mobile phases were 0.1% formic acid in H2O (A) and methanol (B). The flow was 0.8 mL min-1. A Prodigy ODS3 column was used (250 × 4.60 mm, 5 micron, 100 A, Phenomenex, USA). The sample (20 μL) was separated with the next gradient as follows; 0 min (5% B); 4 min (5% B); 40 min (98% B); 43 min (98% B);46 min (5% B); 49 min (5% B). Hydroxytyrosol, tyrosol and verbascoside were quantified by external calibration with the standards. Peaks were identified by retention time, DAD spectra and spiking the sample with pure standards.

Navarro M., Fiore A., Fogliano V, & Morales F.J. (2015). Carbonyl trapping and antiglycative activities of olive oil mill wastewater. Food & function, 6(2).

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