Emodin was obtained from Chenguang Biotechnology Co., LTD (Xi’an, China). Geniposide was purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). L-alpha-phosphatidyl choline (SPC) and cholesterol were acquired from Shanghai Ryon Biological Technology Co. Ltd (Shanghai, China). DSPE-TK-PEG2000 was obtained from Xi’an RuiXi Biological Technology Co., LTD (Xi’an, China). Additionally, 2′,7′-Dichlorofluorescin diacetate was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), MitoSox Red was obtained from MCE (MedChemExpress), and MitoQ was acquired from GLPBIO. Antibodies, including VE-cadherin, cleaved caspase-3, BAX, Bcl-2, and β-actin, were purchased from Proteintech (IL, USA).
Mitosox red
Manufactured by MedChemExpress
Sourced in China, United States
MitoSOX Red is a fluorogenic dye that is highly selective for the detection of superoxide in the mitochondria of live cells. It is a reliable tool for the assessment of mitochondrial oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
Dspe tk peg2000, by Xi’an Ruixi (3 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Cleaved caspase 3, by Proteintech (1 mentions)
Ve cadherin, by Proteintech (1 mentions)
β actin, by Proteintech (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
Bcl 2, by Proteintech (1 mentions)
2 7 dichlorofluorescin diacetate, by Solarbio (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Yoda1, by MedChemExpress (1 mentions)
Fetal bovine serum (fbs), by Yeasen (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Wuhan Servicebio Technology (1 mentions)
Sybr qpcr master mix, by Yeasen (1 mentions)
Fluorescein fitc tunel cell apoptosis detection kit, by Wuhan Servicebio Technology (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Yeasen (1 mentions)
Rhod 2 am, by Yeasen (1 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Yeasen (1 mentions)
H2dcfda, by MedChemExpress (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
5 protocols using mitosox red
1
Emodin-Loaded Nanoparticles for Cellular Responses
2
Measuring Merozoite Oxidative Stress
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mROS was measured using MitoSOX Red (MedChemExpress, Shanghai, China) according to the manufacturer’s instructions. Briefly, after incubation of merozoites with different concentrations of drugs, merozoites from all groups were incubated with 5 μM MitoSOX for 15 min at 37 °C in the dark. After washing 3 times with warm DMEM, we detected the ROS intensity using a fluorescence spectrophotometer (Tecan, Infinite M200 PRO, Männedorf, Switzerland).
3
Fluorescent Reagents for Cell Analysis
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4 kDa FITC-dextran was obtained from Maokang Biotechnology (Shanghai, China). Roswell Park Memorial Institute (RPMI) 1640 medium and Dulbecco’s Modified Eagle Medium (DMEM) was procured from Gibco (USA). Antibodies for Piezo1, Occludin and ZO-1 were procured from Proteintech (China). The following chemicals were obtained from Servicebio (China): fluorescein (FITC) Tunel Cell Apoptosis Detection Kit, Phosphate Buffered Saline (PBS) and Hank’s Balanced Salt Solution (HBSS). Fluorescent-conjugated antibodies for flow cytometry were procured from BD Bioscience (USA). Secondary antibodies conjugated with AlexaFluor-488 or Alexa Fluor 594, Annexin V-FITC /PI Apoptosis Detection Kit, JC-1 Mitochondrial Membrane Potential Assay Kit, SYBR qPCR Master Mix, Rhod-2 AM and Fetal Bovine Serum (FBS) were procured from Yeason (China). Yoda1 and MitoSOx Red were procured from MedChemExpress (USA). MitoTEMPO was acquired from Topscience (China). DMEM no Ca2+ was obtained from Meiluncell (China).
4
Fluorescence-based Mitochondrial and ROS Assays
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Fluorescence-based measurements of mitochondrial membrane potential and intracellular ROS generation were performed as previously described 50 (link). In brief, following treatment, hepatocytes were incubated with the potentiometric JC-1 sensor (Mitochondrial Membrane Potential Detection Kit, #30001, Biotium). For ROS measurements, fresh liver sections or primary hepatocytes were incubated with the cellular ROS indicator DHE (#PD-MY 003, MedChemExpress), cytoplasmic ROS indicator H2DCFDA (#HY-D0940, MedChemExpress), and mitochondrial ROS indicator MitoSOX RED (HY-D1055, MedChemExpress) based on methods provided by manufacturers 51 (link). Fluorescence images were acquired with same laser output and gain for control and treated samples on a Zeiss 880 confocal microscope using a 100X oil objective 52 (link).
5
Ferroptosis-related Molecular Mechanisms
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Erastin (HY-15763), ferrosatatin-1 (HY-100579), N-acetylcystein (HY–B0215) and MitoSOX Red (HY-D1055) were purchased from MedChem Express (Monmouth Junction, NJ). All-trans retinoic acid (ATRA, R2625), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, M2003 ) and 4′,6-diamidino-2-phenylindole (DAPI, D9542 ) were from Merck KGaA (Darmstadt, Germany). Anti-CYTL1 (ab129767) antibody was purchased from Abcam (Cambridge, UK). Anti-CSE (12217-1-AP), anti-ATG5 (10181-2-AP), anti-Keap1 (10503-2-AP), anti-Lamin B1 (12987-1-AP) and anti-p53 (10442-1-AP) antibodies were from Proteintech (Chicago, IL). Anti-HA (3724), anti-LC3B (3868), anti-Myc (2276) and anti-SQSTM1/p62 (8025) antibodies were from Cell Signaling Technology (Beverly, MA). Anti-GAPDH (sc-47724), anti-β-tubulin (sc-5274), anti-β-actin (sc-47778), anti-CBS (sc-133154), anti-Nrf2 (sc-365949), and anti-GPX4 (sc-166570) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Lipofectamine 3000 (L3000), Lipofectamine RNAi MAX (13778), the lysosome-specific dye LysoTraker Red (L7528), and Alexa Fluor 488 goat anti-rabbit IgG (R37116) were purchased from Thermo Fisher Scientific (Waltham, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
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