Log In Sign up
The largest database of trusted experimental protocols

100 and 40 mm nylon cell strainers

Manufactured by BD
Visit Supplier

The 100- and 40-mm nylon cell strainers are laboratory equipment designed for filtration and separation of cells from cell suspensions. They feature a nylon mesh structure that allows the passage of single cells while retaining larger cellular aggregates.

Automatically generated - may contain errors

Lab products found in correlation

Rbc lysis buffer, by Thermo Fisher Scientific (2 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (2 mentions) Clone 2.4g2, by BioXCell (2 mentions) 70 and 40 mm nylon cell strainers, by BD (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dnase 1, by Roche (2 mentions)

2 protocols using 100 and 40 mm nylon cell strainers

1

Isolation of Murine and Human PDAC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse and human PDAC tumors and adjacent pancreata were mechanically dissociated and incubated in collagenase (collagenase II for murine tumors, collagenase IV for human tumors, both 5 mg/ml; Worthington Biochemical Corp., Fisher Scientific), DNAse I (0.5 mg/ml; Roche Diagnostics), and Hank’s balanced salt solution (Gibco, Fisher Scientific) for 30 minutes at 37°C. Digestion was then quenched with fetal bovine serum (FBS, Life Technologies), and cells were filtered sequentially through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific). Tumors, adjacent pancreata, and lymph nodes were then mechanically disassociated and filtered through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS (Life Technologies). Spleens were mechanically dissociated and filtered through 70- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS, followed by RBC lysis (RBC lysis buffer, ThermoFisher Scientific). Mouse Fc receptors were blocked with FcεRIII/II-specific antibody (1 μg per 1 × 106 cells; clone 2.4G2, Bio X Cell).
Moral J.A., Leung J., Rojas L.A., Ruan J., Zhao J., Sethna Z., Ramnarain A., Gasmi B., Gururajan M., Redmond D., Askan G., Bhanot U., Elyada E., Park Y., Tuveson D.A., Gönen M., Leach S.D., Wolchok J.D., DeMatteo R.P., Merghoub T, & Balachandran V.P. (2020). ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity. Nature, 579(7797), 130-135.
+ Open protocol
+ Expand
2

Isolation of Murine and Human PDAC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse and human PDAC tumors and adjacent pancreata were mechanically dissociated and incubated in collagenase (collagenase II for murine tumors, collagenase IV for human tumors, both 5 mg/ml; Worthington Biochemical Corp., Fisher Scientific), DNAse I (0.5 mg/ml; Roche Diagnostics), and Hank’s balanced salt solution (Gibco, Fisher Scientific) for 30 minutes at 37°C. Digestion was then quenched with fetal bovine serum (FBS, Life Technologies), and cells were filtered sequentially through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific). Tumors, adjacent pancreata, and lymph nodes were then mechanically disassociated and filtered through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS (Life Technologies). Spleens were mechanically dissociated and filtered through 70- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS, followed by RBC lysis (RBC lysis buffer, ThermoFisher Scientific). Mouse Fc receptors were blocked with FcεRIII/II-specific antibody (1 μg per 1 × 106 cells; clone 2.4G2, Bio X Cell).
Moral J.A., Leung J., Rojas L.A., Ruan J., Zhao J., Sethna Z., Ramnarain A., Gasmi B., Gururajan M., Redmond D., Askan G., Bhanot U., Elyada E., Park Y., Tuveson D.A., Gönen M., Leach S.D., Wolchok J.D., DeMatteo R.P., Merghoub T, & Balachandran V.P. (2020). ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity. Nature, 579(7797), 130-135.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!