PBMCs were isolated by Ficoll Hypaque (Pharmacia, Piscataway, NJ) with density gradient centrifugation. Purified PBMCs were incubated with or without FAHF-2 (125 or 250 μg/mL) for 24 hours followed by LPS (2 μg/mL) stimulation for additional 24 hours to determine the appropriate concentration to be used in this study.20 (link) Cell viability was determined by trypan blue dye exclusion. Cytokine levels (IL-1β, IL-4, IL-5, IL-6, IL-8, IL-12, IL-13, TNF-α, IFN-γ, and IL-17) in the culture supernatants were determined by enzyme-linked immunosorbent assay (BD Biosciences) or by multiplex immunoassay. Multiplex assays were performed according to the manufacturer’s instructions (Luminex Human assay, Invitrogen, Grand Island, NY).26 (link)
Luminex human assay
Manufactured by Thermo Fisher Scientific
The Luminex Human Assay is a multiplex analytical platform that utilizes color-coded magnetic beads to simultaneously measure multiple analytes from a single sample. The core function of this product is to enable the quantitative detection and analysis of various protein biomarkers, cytokines, and other biomolecules in human biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using luminex human assay
1
PBMC Isolation and Cytokine Profiling
2
Evaluating FAHF-2 Cytokine Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PBMCs were isolated by Ficoll Hypaque (Pharmacia, Piscataway, NJ) with density gradient centrifugation. Purified PBMCs were cultured in medium containing 5% FBS with or without FAHF-2 (250 μg/ml), B-FAHF-2 (60 μg/ml), individual herbs in B-FAHF-2 (doses noted above) or dexamethasone (10−3 μM/ml, positive control) for 24 h followed by LPS (2 μg/ml) stimulation or no stimulation for an additional 24 h (8 (link)). Cytokine, chemokine and growth factor levels in the culture supernatants were determined by ELISA per the manufacturer's instructions (BD Biosciences), by multiplex immunoassay or by cytometric bead array. Multiplex assays were performed per the manufacturer's instructions (Luminex Human assay, Invitrogen, Grand Island, NY). Cytometric bead array was performed per the manufacturer's instructions (BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!