Dual beam uv vis spectrophotometer

Shimadzu dual beam UV–Vis spectrophotometer (Kyoto, Japan), UV-1650 PC model with bundle software. Processing of absorption and derivative spectra was done using version 3.7 of the UV PC personal spectroscopy program (Shimadzu, Kyoto, Japan). Scans have been performed at intervals between 200.0 nm to 400.0 nm at 0.2 nm with 1.00 cm quartz cells.
The CWT was performed using MATLAB^® 9.2.0.538062 (R2017a).

Spectrophotometric measurements were made with a SHIMADZU dual beam UV–Vis spectrophotometer; model UV-160 PC (Kyoto, Japan). The absorbance spectra of the reference and the test solutions were recorded in 1.0-cm quartz cells over the range 200–350 nm at room temperature. A Metrohm 780 digital pH meter (Herisau, Switzerland), Soronex Digitec DT 102H ultrasonicator (Bandelin, Germany) and a universal centrifuge, model premium 20,000 R (Iran), were used in the experiments. All chromatographic measurements were made with a Waters 1500 Series instrument (Milford, MA, U.S.A.) equipped with dual absorbance detector (Waters Model 2487). The separation was carried out on a reversed phase C18 HPLC column (5 µm, 4.6 mm × 250 mm; ODS, Merck, Darmstadt, Germany). The details of the HPLC experiments for determination of SMX and TMP have been explained elsewhere [32 (link)]. Experimental data were processed with programs written in MATLAB (version 6.5, Mathworks, Natick, MA, USA).