Rxi 624sil

Manufactured by Restek

Sourced in United States

The Rxi-624Sil is a capillary gas chromatography column designed for the separation and analysis of a wide range of volatile organic compounds. It features a 624-type stationary phase that provides excellent peak shape and resolution for a variety of analytes, including alcohols, ketones, and halogenated compounds. The column is inert, thermally stable, and suitable for use with a variety of sample types and detection methods.

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Lab products found in correlation

Pegasus 4d, by Leco (5 mentions) Mps autosampler, by Gerstel (4 mentions) Chromatof software version 4, by Leco (3 mentions) Stabilwax secondary column, by Restek (3 mentions) Stabilwax, by Restek (2 mentions) Agilent 6890 gc, by Agilent Technologies (1 mentions) Agilent 7890 gc, by Agilent Technologies (1 mentions) Carboxen 1000, by Merck Group (1 mentions) Tedlar bag, by SKC (1 mentions) Chromatof software, by Leco (1 mentions)

Close spelling variants of this product

Rxi-624Sil MS Rxi-624Sil MS column Rxi-624Sil column

5 protocols using rxi 624sil

Comprehensive Volatile Analysis by GC×GC-TOF MS

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The analyses of volatile molecules were carried out using a Pegasus 4D (LECO Corp., St. Joseph, MI, United States) GC×GC time-of-flight (TOF) MS instrument with an Agilent 6890 GC, and equipped with an MPS autosampler (Gerstel, Linthicum Heights, MD, United States). The first dimension column was an Rxi-624Sil (60 m × 250 μm × 1.4 μm (length × internal diameter × film thickness)) connected in series with a Stabilwax secondary column (1 m × 250 μm × 1.4 μm), both from Restek (Bellefonte, PA, USA). The carrier gas was helium, at a flow rate of 2 mL/min. The primary oven temperature program was 35°C (hold 1 min) ramped to 230°C at a rate of 3.5°C/min. The secondary oven and the thermal modulator were offset from the primary oven by +5°C and +25°C, respectively. A modulation period of 2.0 s (alternating 0.5 s hot and 0.5 s cold pulses) was used. The transfer line temperature was set at 250°C. A mass range of m/z 30 to 500 was collected at a rate of 200 spectra/s following a 2.5 min acquisition delay. The ion source was maintained at 200°C. Data acquisition and analysis were performed using ChromaTOF software, version 4.50 (LECO Corp).

Purcaro G., Rees C.A., Melvin J.A., Bomberger J.M, & Hill J.E. (2018). VOLATILE FINGERPRINTING OF PSEUDOMONAS AERUGINOSA AND RESPIRATORY SYNCYTIAL VIRUS INFECTION IN AN IN VITRO CYSTIC FIBROSIS CO-INFECTION MODEL. Journal of breath research, 12(4), 046001.

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GC×GC-TOF MS Volatilome Analysis

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The TD tubes were desorbed into a Pegasus 4D (LECO Corporation, St. Joseph, MI) GC × GC-TOF MS instrument with an Agilent 7890 GC equipped with a TD unit, cooled injection system (CIS), and a Multi-Purpose Sampler (MPS) autosampler (Gerstel, Linthicum Heights, MD). The solvent venting time was 10 min (30 °C; 60 ml min⁻¹), cryofocusing time was 5 min (−100 °C), sample desorption time was 180 s, CIS temperature was 330 °C, and the injection mode was splitless. Chromatographic analysis was performed using an Rxi-624Sil (60 m × 250 μm × 1.4 μm) as the first dimension (1D)-GC column and a Stabilwax (1.5 m × 250 μm × 0.5 μm) as second dimension (2D)-GC column, both purchased from Restek (Bellefonte, PA, US). The modulation time was 2 s total and helium as the carrier gas (flowrate: 2 ml min⁻¹). TOF MS was employed as a detector, with the following parameters: electron impact at 70 eV; acquisition range: 30–500 mz⁻¹; acquisition rate: 200 spectra/s; and ion source temperature: 200 °C. Data acquisition and analysis was performed using ChromaTOF software, version 4.50 (LECO Corporation).

Beccaria M., Bobak C., Maitshotlo B., Mellors T.R., Purcaro G., Franchina F.A., Rees C.A., Nasir M., Black A, & Hill J.E. (2018). Exhaled human breath analysis in active pulmonary tuberculosis diagnostics by comprehensive gas chromatography-mass spectrometry and chemometric techniques. Journal of breath research, 13(1), 016005.

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Comprehensive GC×GC-TOF-MS Analysis of Complex Samples

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A Pegasus 4D (LECO Corporation, St. Joseph, MI) GC ×GC time-of-flight (TOF) MS instrument with an Agilent 6890N GC, and an MPS autosampler (Gerstel, Linthicum Heights, MD, USA) equipped with a cooled sampler tray (4 °C), was used. The primary column was an Rxi-624Sil (60 m ×250 μm ×1.4 μm) connected in series with a Stabilwax secondary column (1 m ×250 μm ×1.4 μm) from Restek (Bellefonte, PA, USA). The carrier gas was helium, at a flow rate of 2 ml min⁻¹. The primary oven temperature program was 35 °C (hold 1 min) ramped to 230 °C at a rate of 5 °C min⁻¹. The secondary oven and the thermal modulator were offset from the primary oven by +5 °C and +25 °C, respectively. A modulation period of 2.5 s (alternating 0.75 s hot and 0.5 s cold) was used. The transfer line temperature was set at 250 °C. A mass range of m/z 30 to 500 was collected at a rate of 200 spectra/s following a 3 min acquisition delay. The ion source was maintained at 200 °C. Data acquisition and analysis was performed using ChromaTOF software, version 4.50 (LECO Corp.).

Purcaro G., Rees C.A., Wieland-Alter W.F., Schneider M.J., Wang X., Stefanuto P.H., Wright P.F., Enelow R.I, & Hill J.E. (2018). Volatile fingerprinting of human respiratory viruses from cell culture. Journal of Breath Research, 12(2), 026015.

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GC-GCxToF-MS Analysis of Breath Volatiles

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One liter Tedlar bags (SKC Inc, Eighy Four, PA, US) were used for the collection of breath [22] .
A three-bed TD tube containing Carbopack Y, X, and Carboxen 1000 (Supelco, Bellefonte, PA), a sorbent combination previously optimized for the collection of a wide range of breath molecules, was used to concentrate and store volatile molecules [15] . TD tubes were desorbed in a Pegasus 4D (LECO Corporation, St. Joseph, MI) GC×GC-ToFMS instrument with an Agilent 7890 GC equipped with a TDU, CIS, and MPS autosampler (Gerstel, Linthicum Heights, MD). Solvent venting time: 10 min (30°C; 60 mL/min); cryofocusing time: 5 min (-100°C), sample desorption time: 180s; CIS temperature: 330°C; injection mode: splitless. Chromatographic analysis was performed using a Rxi-624Sil (60m×250μm×1.4μm) as 1 D-GC column and a Stabilwax (1.5m×250μm×0.5μm) as 2 D-GC column, both purchased from Restek (Bellafonte, PA, US).
Modulation time was 2 sec total and helium as carrier gas (flow-rate: 2 mL/min). For detecting breath molecules, a ToFMS was employed, with the following parameters: electron impact at 70 eV; acquisition range: 30-500 m/z; acquisition rate: 200 spectra/s; ion source temperature: 200°C.

Beccaria M., Mellors T.R., Petion J.S., Rees C.A., Nasir M., Systrom H.K., Sairistil J.W., Jean-Juste M.A., Rivera V., Lavoile K., Severe P., Pape J.W., Wright P.F, & Hill J.E. (2018). Preliminary investigation of human exhaled breath for tuberculosis diagnosis by multidimensional gas chromatography - Time of flight mass spectrometry and machine learning. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1074-1075.

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GC×GC-TOF-MS Analysis of Complex Samples

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A Pegasus 4D (LECO Corporation, St. Joseph, MI) GC×GC time-of-flight (TOF) MS instrument with an Agilent 7890 GC, and an MPS autosampler (Gerstel, Linthicum Heights, MD, USA) equipped with a cooled sampler tray (4 °C), was used. The primary column was an Rxi-624Sil (60 m × 250 µm × 1.4 µm) connected in series with a Stabilwax secondary column (1 m × 250 µm × 1.4 µm) from Restek (Bellefonte, PA, USA). The carrier gas was helium, at a flow rate of 2 mL/min. The primary oven temperature program was 35 °C (hold 1 min) ramped to 230 °C at a rate of 5 °C/min. The secondary oven and the thermal modulator were offset from the primary oven by +5 °C and +25 °C, respectively. A modulation period of 2.5 s (alternating 0.75 s hot and 0.5 s cold) was used. The transfer line temperature was set at 250 °C. A mass range of m/z 30 to 500 was collected at a rate of 200 spectra/s following a 3 min acquisition delay. The ion source was maintained at 200 °C. Data acquisition and analysis was performed using ChromaTOF software, version 4.50 (LECO Corp.).

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