Chicken (Gallus gallus domesticus) eggs (Winter Egg Farm, Cambridge, UK) were incubated in a digital cabinet incubator (OVA Easy 380, Brinsea) at 38 degrees. At Hamburger Hamilton developmental stage 19 (HH19), the eggshell was fenestrated, the window covered with parafilm (VWR) and eggs were placed horizontally in the incubator. At HH35, epicardial cells were transplanted in PSC with matrigel (1:2 dilution) onto the chorionic chicken vasculature. HESCs were fully differentiated to epicardial cells before administration into the chicken embryos. The eggs were then returned in the incubator until stage HH40. The matrigel plugs were harvested and fixed in 4% paraformaldehyde before being stained with anti-HLA1 (abcam) anti-SM22alpha (Abcam) and sambucus nigra lectin (Vector laboratories).
Anti hla1
Manufactured by Abcam
Anti-HLA1 is an antibody product used in laboratory research applications. It targets the HLA-A antigen, which is a key component of the major histocompatibility complex class I. The antibody can be used to detect and study the expression of HLA-A in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti hla1
1
Epicardial Cell Transplantation in Chicken Embryos
2
Epicardial Cell Transplantation in Chicken Embryos
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Chicken (Gallus gallus domesticus) eggs (Winter Egg Farm, Cambridge, UK) were incubated in a digital cabinet incubator (OVA Easy 380, Brinsea) at 38 degrees. At Hamburger Hamilton developmental stage 19 (HH19), the eggshell was fenestrated, the window covered with parafilm (VWR) and eggs were placed horizontally in the incubator. At HH35, epicardial cells were transplanted in PSC with matrigel (1:2 dilution) onto the chorionic chicken vasculature. HESCs were fully differentiated to epicardial cells before administration into the chicken embryos. The eggs were then returned in the incubator until stage HH40. The matrigel plugs were harvested and fixed in 4% paraformaldehyde before being stained with anti-HLA1 (abcam) anti-SM22alpha (Abcam) and sambucus nigra lectin (Vector laboratories).
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