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Lsr flow cytometer

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The LSR flow cytometer is a laboratory instrument used for the analysis and sorting of cells or particles in a fluid suspension. It is capable of detecting and measuring multiple physical and fluorescent characteristics of individual cells or particles as they pass through a laser beam.

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Lab products found in correlation

Cd49b pe, by BD (2 mentions) Live dead amcyan, by Thermo Fisher Scientific (2 mentions) Cd69 af700, by BD (2 mentions) Ack lysing buffer, by Thermo Fisher Scientific (2 mentions) Cd11c pe cy7, by Thermo Fisher Scientific (1 mentions) Ifn γ apc cy7, by BD (1 mentions) Cd86 fitc clone gl1, by BD (1 mentions)

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LSR-II/Fortessa flow cytometer (6 citations)

5 protocols using lsr flow cytometer

1

Multicolor Flow Cytometry Analysis of Bone Marrow Myeloid Cells

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BMMs were incubated in ACK lysing buffer (Thermo Fisher Scientific) for 5 minutes and washed with Hanks’ balanced salt solution containing 10 mM HEPES (pH 7.4) and 2% FBS. Cells were incubated with fluorochrome-conjugated antibodies: anti-CD3, anti-CD45R (B220), anti-CD11b (Mac-1), anti-CD117 (c-kit) and anti-CD115 (c-Fms) for 30 min on ice. All antibodies were purchased from eBioscience. After washing, cells were analyzed using a LSR flow cytometer and FlowJo software (Tree Star).
Shin B., Kupferman J., Schmidt E., Polleux F., Delany A.M, & Lee S.K. (2020). Rac1 inhibition via Srgap2 restrains inflammatory osteoclastogenesis and limits the clastokine, SLIT3. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 35(4), 789-800.
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2

Flow Cytometry Analysis of Lung Immune Cells

Cited 1 time
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The prepared lung and BAL cells were stained with different surface markers. The cell surface markers for flow cytometry analysis included CD11c+CD11b−F4/80+MHCIIhi (alveolar macrophage), F4/80−DX5+CD69+ (natural killer Cells), F4/80−DX5+CD69+(activated natural killer cells), and CD11c−CD11b+siglecF−Ly6chiF4/80+ (monocytes) as described48 (link). Stained cell samples were performed by Becton-Dickenson LSR flow cytometer and data analysis by Flowjo Software Program (Tree Star Inc.).
Jung Y.J., Lee Y.T., Ngo V.L., Cho Y.H., Ko E.J., Hong S.M., Kim K.H., Jang J.H., Oh J.S., Park M.K., Kim C.H., Sun J, & Kang S.M. (2017). Heat-killed Lactobacillus casei confers broad protection against influenza A virus primary infection and develops heterosubtypic immunity against future secondary infection. Scientific Reports, 7, 17360.
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3

Multiparametric flow cytometry of lung immune cells

Cited 3 times
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Lung cells were prepared by Percoll gradient (44% and 67%) followed by homogenization. To analyze the immune cell populations in mucosal sites, the prepared lung and BALF cells were stained with different cell surface marker antibodies to analyze the expression of antigen presenting cells (APCs) and germinal center (GC) B cells; monocytes (CD11c−CD11b+siglecF-Ly6chi F4/80+), neutrophils (CD11c− CD11b+ siglecF+-Ly6c + F4/80− ), and GC B cells (CD19+IgD−GL7+B220+). For APCs staining, CD45-PerCP, CD11b-APC, CD11c-PECy7, CD103-Pacific Blue, F4/80-FITC, Ly6c-A700, SiglecF-PE, and MHCII-PECy5 were used. CD3-e450, CD19-PECy5.5, IgD-PE, B220-APCCy7, GL7-FITC, CD138-APC were stained to analyze germinal center B cells [11 (link), 14 , 22 (link), 23 (link)]. The data were acquired by Becton-Dickenson LSR flow cytometer and analyzed by FlowJo software Program (Tree Star Inc., Ashland, OR, USA).
Jung Y.J., Kim K.H., Ko E.J., Lee Y., Kim M.C., Lee Y.T., Kim C.H., Jeeva S., Park B.R, & Kang S.M. (2020). Adjuvant effects of killed Lactobacillus casei DK128 on enhancing T helper type 1 immune responses and the efficacy of influenza vaccination in normal and CD4-deficient mice. Vaccine, 38(36), 5783-5792.
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4

NK Cell and Dendritic Cell Co-culture Assay

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Resting NK cells (2 × 105 cells/mL) and mDCs (1 × 106 cells/mL) were co-cultured at a ratio of 1:5 in the 96-well U-bottomed plate for 2 d. The cultured cells were then harvested, and the supernatant was stored for cytokine ELISA. Then, the cells were resuspended in FACS buffer, which was stained with different surface markers, including Live/Dead-Amcyan (Thermo Fisher Scientific), CD49b-PE (BD PharmingenTM), CD69-AF700 (activation marker for NK cells; BD PharmingenTM), CD11c-PE/Cy7 (Thermo Fisher Scientific), CD40-BV605 (Thermo Fisher Scientific), and intracellular cytokine marker IFN-γ-APC/Cy7 (BD PharmingenTM). The cell phenotypes and the frequency of IFN-γ were determined using the Becton-Dickenson LSR flow cytometer and analyzed using the Flowjo Software Program (Tree Star Inc., OR, USA).
Le C.T., Ahn S.Y., Kang S.M, & Ko E.J. (2021). Functional NK Cell Activation by Ovalbumin Immunization with a Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Dendritic Cell Maturation. Vaccines, 9(10), 1061.
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5

Immature DC and ANK Cell Co-culture

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Immature DCs (iDCs, 2 × 105 cells/mL) and ANK cells (4 × 105 cells/mL) were co-cultured at a ratio of 1:2 in the 96-well U-bottomed plate. After 2 d of incubation, the cultured cells were harvested. The supernatant was stored for cytokine ELISA and the cells were resuspended in FACS buffer and stained with different surface markers, including Live/Dead-Amcyan (Thermo Fisher Scientific), CD49b-PE (BD PharmingenTM), CD69-AF700 (BD PharmingenTM, Franklin Lakes, NJ, USA), CD11c-PE/Cy7 (Monoclonal Antibody N418; Thermo Fisher Scientific, Waltham, MA, USA), CD40-BV605 (clone 3/23; BD Biosciences, Franklin Lakes, NJ, USA), and CD86-FITC (clone GL1; BD PharmingenTM). Flow cytometry data were acquired using a Becton-Dickenson LSR flow cytometer and analyzed using the Flowjo Software Program (Tree Star Inc., Ashland, OR, USA).
Le C.T., Ahn S.Y., Kang S.M, & Ko E.J. (2021). Functional NK Cell Activation by Ovalbumin Immunization with a Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Dendritic Cell Maturation. Vaccines, 9(10), 1061.
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