Absorption spectra were taken with an Agilent 8453 UV-visible spectrometer. Steady-state photoluminescence measurements were recorded on an Edinburgh Instruments FLS980 fluorimeter. PLQE was measured using a 520 nm CW laser and the sample inside an integrating sphere, with detection via a fibre optic cable to an Andor iDus DU420A BVF Si detector. PLQE was calculated by the de Mello method to account for secondary absorption of scattered light50 (link). For photoluminescence measurements in a magnetic field, we used the CW 520 nm source and a pair of lenses to project the photoluminescence emitted to a solid angle of 0.1π onto an InGaAs detector, with the sample positioned in an electromagnet (GMW—Model 3470) with 3 cm distance between cylindrical poles. The magnetic field for a given voltage supplied to the electromagnet was measured with a gauss metre. Photostability measurements were performed using the same detector and excitation, with the transmitted 520 nm laser signal being measured over time, and converted to absorption using Absorbed photons = Incident photons−Transmitted photons.
8453 uv visible spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The 8453 UV-visible spectrometer is a compact, high-performance instrument designed for routine analysis in a laboratory setting. It measures the absorption of light in the ultraviolet and visible regions of the electromagnetic spectrum, providing data on the chemical composition and concentration of samples. The 8453 offers fast, accurate, and reliable results for a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Fls980 fluorimeter, by Edinburgh Instruments (1 mentions)
2400 chn elemental analyzer, by PerkinElmer (1 mentions)
Jobin yvon fluorolog3 spectrometer, by Horiba (1 mentions)
Absolute pl quantum yield spectrometer, by Hamamatsu Photonics (1 mentions)
D8 advance, by Bruker (1 mentions)
Flsp920 spectrometer, by Edinburgh Instruments (1 mentions)
7 protocols using 8453 uv visible spectrometer
1
Characterization of Optoelectronic Materials
2
Ferroxidase Activity Monitoring in Ferritin
3
Comprehensive Analytical Techniques for Research
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Electrochemical experiments were carried
out using a Biologic multichannel VSP 201 potentiostat. Gas phase
analysis of H2 was carried out using a GOW MAC gas chromatograph,
with a thermal conductivity detector and two columns in series (4’X1/8’’
St. St. Hayesep T, 10’X1/8’’ St. St., Molecular
sieve SA) with Ar as a gas carrier. UV–vis measurements were
done using an Agilent 8453 UV–visible spectrometer with deuterium
and tungsten lamps as light sources. IR measurements were carried
out on a Nicolet 5700 FTIR instrument. Mass spectrometry measurements
were done with a Xevo G2-XS QTOF high resolution ESI TOF MS instrument.
NMR measurements were done with a Bruker AVANCE III HD-500 MHz magnet.
Thermo gravimetric analysis was measured using an SDT Q 600 using
alumina crucibles. ICP-MS analysis was carried out using an Agilent
7700s spectrometer.
out using a Biologic multichannel VSP 201 potentiostat. Gas phase
analysis of H2 was carried out using a GOW MAC gas chromatograph,
with a thermal conductivity detector and two columns in series (4’X1/8’’
St. St. Hayesep T, 10’X1/8’’ St. St., Molecular
sieve SA) with Ar as a gas carrier. UV–vis measurements were
done using an Agilent 8453 UV–visible spectrometer with deuterium
and tungsten lamps as light sources. IR measurements were carried
out on a Nicolet 5700 FTIR instrument. Mass spectrometry measurements
were done with a Xevo G2-XS QTOF high resolution ESI TOF MS instrument.
NMR measurements were done with a Bruker AVANCE III HD-500 MHz magnet.
Thermo gravimetric analysis was measured using an SDT Q 600 using
alumina crucibles. ICP-MS analysis was carried out using an Agilent
7700s spectrometer.
4
Estimating Protein Concentration via UV-Vis
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The prepared HEWL stock was diluted ten times using MilliQ water. The UV-visible absorption spectrum of this solution was obtained using Agilent 8453 UV-Visible spectrometer, Santa Clara, CA, USA, and the absorption value at 280 nm estimated the protein concentration of the stock with the extinction coefficient of 37970 M−1cm−1 using the following equation [59 (link)]:
Concentration of protein = ((Absorbance at 280 nm × 107)/37,970) μM
Concentration of protein = ((Absorbance at 280 nm × 107)/37,970) μM
5
Measuring Tartrazine Release by UV-VIS
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The tartrazine released from the films was measured with an ultraviolet-visible (UV-VIS) spectrometer (8453 UV-visible spectrometer, Agilent, USA) at 425 nm. The medium used was 0.9 % NaCl solution, with the same technique used for evaluation of the release samples.
6
Photophysical Characterization of Pd3O3 in PMMA
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The photoluminescent spectra were measured on a Horiba Jobin Yvon FluoroLog-3 spectrometer. The absorption spectrum was measured on an Agilent 8453 UV-visible spectrometer. Doped thin films for photoluminescent quantum yield (PLQY) measurements were fabricated by the drop-cast process of Pd3O3 in a PMMA host. The PLQY measurements were carried out on a Hamamatsu absolute PL quantum yield spectrometer model C11347. It should be noted that the error bar may be large for the PLQY measurement with emissive materials which have long lifetimes (>100 μs) due to quenching by residual oxygen in the integration sphere. Cyclic voltammetry and differential pulsed voltammetry were performed on a CHI610B electrochemical analyzer in a solution of anhydrous DMF using 0.1 M tetra(nbutyl) ammonium hexafluorophosphate as the supporting electrolyte. A ferrocene/ferrocenium (Fc/Fc+) redox couple used as an internal reference. The HOMO and LUMO values were determined following literature reported fits relating the electrochemical potentials to the values determined via ultraviolet photoemission spectroscopy and inverse photoelectron spectroscopy, respectively.42 ,43 Mass spectra were recorded on JEOL GCmate gas chromatograph/mass spectrometer. Elemental analysis was carried out with a Perkin-Elmer 2400 CHN Elemental Analyzer.
7
Structural Characterization of CsPbBr3 Perovskite NCs
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Crystal structure identification of the NCs was carried out using XRD, performed on a Bruker D8 Advance diffractometer using Cu-Kα radiation. The bulk XRD pattern was obtained from the inorganic crystal structure database (ICSD). TEM measurements were performed using a Tecnai F30UHR version electron microscope, equipped with a field emission gun (FEG) at an accelerating voltage of 200 kV and a JEOL JEM-3010 transmission electron microscope with an accelerating voltage of 300 kV. A drop of purified NCs which were dissolved in hexane was placed on a carbon coated Cu grid. The solvent was allowed to evaporate leaving behind the NCs for imaging. UV-visible absorption spectra of CsPbBr3 perovskite NCs dissolved in hexane were obtained using an Agilent 8453 UV-visible spectrometer. Steady state PL spectra were collected using a 450 W xenon lamp as the light source on an FLSP920 spectrometer, Edinburgh Instruments, while the PL lifetime measurements were carried out on the same instrument using an EPL-405 pulsed diode laser as the excitation source (λex = 405 nm).
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