The largest database of trusted experimental protocols

16 protocols using silver wire

1

Cytochrome c Purification and Electrochemical Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytochrome c from equine heart (ref. C7752: MW~12.4 kDa, purity ≥ 95%), acetic acid (glacial), and Dulbecco’s Phosphate Buffered Saline (pH 7.4) were purchased from Sigma-Aldrich (Buchs, Switzerland). Sodium chloride and sodium acetate were obtained from Fisher Scientific (Loughborough, UK) and Fluka (Buchs, Switzerland), respectively.
Silver wire and silver chloride (AgCl) for the preparation of electrodes were obtained from Sigma Aldrich (Steinheim, Germany). PVC tubing (3 mm ID, 5 mm OD, and 1 mm wall) and agarose (used to prepare salt bridge assemblies) were obtained from VWR International AG (Dietikon, Switzerland) and Conda (Madrid, Spain), respectively.
The organic solvents, acetonitrile (ACN) and methanol (MeOH), were HPLC grade (Fischer Scientific, Loughborough, UK). Trifluoroacetic acid (TFA; 99% extra pure) was obtained from Acros organics (Geel, Belgium). Ultra-pure water (Millipore Milli-Q Gard 1 Purification pack resistivity > 18 MΩ.cm; Zug, Switzerland) was used to prepare all solutions. All other chemicals were at least of analytical grade.
+ Open protocol
+ Expand
2

Iontophoretic Transdermal Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lidocaine hydrochloride, silver wire (0.5 mm diameter), and silver chloride used for preparation of electrodes were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, methanol, potassium phosphate monobasic (KH2PO4), and PBS (phosphate buffered saline) were purchased from Fisher Scientific (NJ, USA). Transpore tape for tape stripping was obtained from 3 M (St. Paul, MN, USA). Deionized water was used to prepare all the solutions required in this study and for HPLC analysis. Iontophoresis power supply unit (Model 6221) was purchased from Keithley Instruments (Cleveland, OH, USA).
+ Open protocol
+ Expand
3

Carbon-Fiber Microelectrodes for Dopamine Sensing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Carbon-fiber microelectrodes were fabricated as previously described [37 (link)]. T-40 carbon fibers (Cytec Thornel, Woodland Park, NJ) were first aspirated into 0.68 mm I.D. glass capillary (A-M Systems, Inc., Sequim, WA). Capillaries were then heated and pulled to form a seal around the fiber using a type PE-2 pipette puller (Narishige, Tokyo, Japan). Fibers were cut 70 – 80 μm in length from the glass seal. These electrodes were coated with PEDOT:Nafion following procedures described by Vreeland et al. [38 (link)] to minimize bio-fouling and increase electrochemical selectivity and sensitivity. Reference electrodes (Ag/AgCl) were prepared by soaking a silver wire (0.5 mm diameter, Sigma-Aldrich, St. Louis, MO) in chlorine bleach for 24 hours.
Electrodes were calibrated using a flow cell and known concentrations of dopamine. A calibration factor of 22 nA/μM, was used to convert current measurements to dopamine concentration.
+ Open protocol
+ Expand
4

Fabrication of Electrochemical Sensor

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chloroplatinic acid hexahydrate, 3-aminobenzoic acid (ABA), lead(II) acetate trihydrate, iron(II) sulfate heptahydrate, potassium dioxide, dimethyl sulfoxide (DMSO), silver wire (250 μm in diameter), and platinum wire (76 μm in diameter) were purchased from Sigma (St. Louis, MO, USA). Tungsten wire (100 μm in diameter) was purchased from Alfa Aesar (Ward Hill, MA, USA). Calcium chloride and silver conducting epoxy paste were products of Junsei (Tokyo, Japan) and ASAHI (Tokyo, Japan), respectively. Methyltrimethoxysilane (MTMOS) and (Heptadecafluoro-1,1,2,2-tetrahydrodecyl) trimethoxysilane (17FTMS) were purchased from Fluka (Buchs, Switzerland) and Gelest (Tullytown, PA), respectively. Nitric oxide (99.99% and 87,500 ppm) and nitrogen (99.99%) gases were purchased from Dong-A Scientific (Seoul, Korea). Other solvents and chemicals were analytical-reagent grade. Distilled water of 18.2 MΩ cm resistivity was used for preparing all aqueous solutions.
+ Open protocol
+ Expand
5

Rapid Pathogen Detection via RPA

Check if the same lab product or an alternative is used in the 5 most similar protocols
We purchased the TwistAmp Basic RPA kit and
Oligonucleotide primers from TwistDX Limited (Cambridge, U.K.) and
Eurofins Genomics Europe Shared Services GmbH (Germany), respectively.
The stem-loop oligonucleotide probe was customized by Integrated DNA
Technologies, Inc. (IDT, Iowa, USA). S. epidermidis ATCC 12228 was purchased from ATCC LGC Standards (USA). Lambda exonuclease
enzyme, phosphate-buffered saline (PBS) tablets pH 7.4, nuclease-free
water, and 10× Tris-borate-EDTA (TBE) buffer were purchased from
Fisher Scientific (Sweden). Gold wire of 100 μm diameter (99.99%
trace metals basis), silver wire of 100 μm diameter (99.99%
trace metals basis), Tris (2-carboxyethyl) phosphine hydrochloride
(TCEP), 6-mercapto-1-hexanol (6-hydroxy-1-hexanethiol, 97%), and sulfuric
acid (99.999%) were purchased from Sigma-Aldrich (Sweden). The QIAamp
DNA kit (QIAGEN, Germany) was used for the extraction and purification
of genomic DNA.
+ Open protocol
+ Expand
6

Fabrication and Characterization of Hybrid Supercapacitor Electrodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Indium tin oxide glasses (ITO, 7 Ω/sq) were obtained from Xin Yan Technology Limited. N, N-dimethylformamide (DMF) were supplied by Merck. AC, mesoporous carbon, polytetrafluoroethylene (PTFE), polyacrylonitrile (PAN, Mw = 150,000), silver wire (Ag, diam. 0.5 mm), 3, 4-ethylenedioxythiophene (EDOT, 97% purity), lithium perchlorate (LiClO4, ≥97% purity) and manganese (II) sulfate monohydrate (MnSO4•H2O) were purchased from Sigma-Aldrich. KCl and sulphuric acid (H2SO4) were acquired from Fisher Scientific. Acetone and ethanol were supplied from HmbG Chemicals and J. Kollin Chemicals, respectively. Deionised water (18.2 MΩ.cm) was used throughout the experiments. All chemicals were directly used as obtained without further purification.
+ Open protocol
+ Expand
7

Electrochemical Sensing of Nitric Oxide

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ruthenium chloride (III), bdqi (1,2 benzoquinonediimine), 2,2′:6′,2′′-terpiridine, silver chloride (99.99%), platinum wire, silver wire (0.5 cm diameter) obtained from Sigma-Aldrich, St Louis, MO, USA; Hepes (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and methanol were obtained from J.T. Baker, Center Valley, PA, USA; NO detection full commercial kit (ADI-917-020) from Emzo Life Sciences, Farmingdale, NY, USA; ethanol was obtained from Vetec, Duque de Caxias, RJ, Brazil. All solutions used were prepared from a reverse osmosis water purification system. All other chemical reagents were of analytical grade.
+ Open protocol
+ Expand
8

Microneedle-Assisted Growth Hormone Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microneedle devices (Tappy Tok Tok ®) were developed and supplied by U-biomed, Inc. (Daegu, Korea). rhGH was obtained from Dong-A ST, Inc. (Yong-in, Korea). Silver wire was purchased from Sigma-Aldrich (St. Louis, MO, USA). rhGH enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D systems, Inc. (Minneapolis, MN, USA). All other chemicals and solvents were of reagent grade.
+ Open protocol
+ Expand
9

Electrochemical Sensor Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyvinylidene fluoride (PVDF), silver wire, imidacloprid and clothianidin were purchased from Sigma-Aldrich and used as received. Deionized water (18.2 MΩ cm) from an ELGA Pure Lab Ultra system was used for the preparation of samples.
+ Open protocol
+ Expand
10

Nerve Conduction Analysis Platform

Check if the same lab product or an alternative is used in the 5 most similar protocols
The NC was designed in Solidworks 2020 (Solidworks Corp., USA) and built from a photopolymer resin using a 3D printer (Objet Eden260VS, Stratasys). Figure 2 shows the printed NC. The chamber can hold nerves of different thicknesses and lengths. In the middle of the NC is a circular section that slots over a stainless steel plate with a precisely cut pinhole of 0.5 or 1 mm diameter (P500D or P1000K, Thorlabs, Germany) to constrain the light beam to a well-defined diameter. For electrical stimulation and recording of CNAPs, five electrodes, hosted in grooves, were used (99.99% silver wire, diameter 250 μm, Sigma Aldrich, Belgium). Two of them for stimulation (anode and cathode) and the remaining three for recording (GND: ground, CNAP+ and CNAPÀ). The distance between the electrodes of the same group ranged from 1.4 to 3 mm. For lobster nerves, the distance between cathode and CNAP+ was 30 mm. For rat sciatic nerves, this value was 25 mm.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!