Antibodies against cox2 and β actin

Acetonitrile of HPLC grade was purchased from TEDIA (Fairfield, OH), and analytical reagent grade trifluoroacetic acid was purchased from Nanjing Chemical Reagent Company (Nanjing, China). Ultrapure water was prepared in the lab using a Millipore water purification system (Milford, MA). Paeoniflorin (PubChem ID 442534, purity >98%) and amygdalin (PubChem ID 656516, purity >98%) were purchased from MedChem Express, and paeonol (PubChem ID 24895579, purity >99%) and cinnamaldehyde (PubChem ID 24900954, purity >95%) were purchased from Sigma Aldrich (Shanghai, China).
Antibodies against COX2 and β-actin were purchased from Santa Cruz. HRP-conjugated secondary antibodies were purchased from Sigma Aldrich. The ECL reagent kit was purchased from Thermo Fisher. A COX2 inhibitor screening kit was purchased from Beyotime Technologies (#S0168, Nantong, China).

Dulbecco-modified Eagle medium (DMEM) and Lipofectamine 2000 transfection reagent were obtained from Invitrogen Life Technologies (Grand Island, NY, USA). Fetal bovine serum (FBS) was purchased from GIBCO (Burlington, ON, USA). ART was purchased from Bide Pharmaceutical Corporation (Guangzhou, China). PGE₂ was purchased from Sigma Aldrich (St Louis, MO, USA). The human PGE₂ ELISA kit was obtained from Uscn Life Science Inc (Wuhan, China). Antibodies against COX-2 and β-Actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The Detergent Compatible (DC) Protein Assay kit was purchased from Bio-Rad Laboratories (Hercules, CA, USA).

Chemical standards for danshensu, protocatechuic aldehyde, salvianolic acid A, salvianolic acid B, salvianolic acid D rosmarinic acid, lithospermic acid, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA were purchased from Chengdu Must Bio-Tech Co. Ltd. (China). Ginsenosides Rg1, Rb1, Rh1, and Rd were purchased from Jilin University (China). Isolithospermic acid A, isolithospermic acid B and salvianolic acid G were isolated from Salvia miltiorrhiza in the authors’ laboratory. Their chemical structures (Supplementary Figure S1) were unambiguously identified by comparison of the NMR and MS spectra data with reported literatures. The purity of each reference compound was determined to be higher than 98% by normalization of the peak areas detected by HPLC-UV. LPS from Escherichia coli 055:B5 was purchased from Sigma (USA). Antibody against iNOS was obtained from Cell Signaling Technology (USA); antibodies against COX-2 and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).