Q505lp

FigureS4 presents the apparatus we used for high-throughput sizing and quantitation of DNA. The apparatus consisted of a pressure chamber, a pL-to-fL sample injection scheme, a bare narrow open capillary column, and a laser-induced fluorescence (LIF) detector. As pressurized air was introduced to the pressure chamber, the eluent in the solution vial was pressurized into the BaNC-HDC system. A pL-to-fL sample containing a few to a few hundred of DNA molecules was injected into the narrow capillary column for BaNC-HDC separations. The resolved DNA fragments were monitored by the LIF detector.
The LIF detector was built in-house. Briefly, a 488-nm laser beam from an argon ion (Spectra-Physics, Salt Lake City, UT, USA) was reflected by a dichroic mirror (Q505LP, Chroma Tech-nology, Rockingham, VT, USA) and focused onto the separation capillary through an objective lens (206 and 0.5 NA, Rolyn Optics, Covina, CA, USA). The fluorescence from the narrow capillary was collimated by the same objective lens, passed through the dichroic mirror, a band-pass filter (532 nm), and a 2-mm pinhole, and finally collected by a photosensor module (H5784-01, Hamamatsu, Japan). The amplified signal from the photosensor module was acquired by an ADC card DAQCard-6062E (National Instrument, Austin, TX, USA) and processed using an in-laboratory written LabView program.