3h norepinephrine

N-Ethylnorpentylone reference material was purchased from Cayman Chemical (Ann Arbor, MI, USA) and MDMA-d₅, used as internal standard (IS), was purchased from Cerilliant (Round Rock, TX, USA). Methanol and formic acid (98-100%) were purchased from Scharlau (Barcelona, Spain). Ammonium formate and tert-butyl methyl ether (MTBE) were purchased from Sigma-Aldrich (St Louis, MO, USA) and sodium tetraborate from LS Chemicals (Ribeirao Preto, SP, Brazil). Ultrapure deionized water was purified by a Milli-Q RG unit from Millipore (Billerica, MA, USA). All solvents employed in the sample extractions were HPLC grade. N-Ethylnorpentylone, pentylone and methylone used for monoamine transporter assays were obtained from Cayman Chemical. [³H]Norepinephrine (60 Ci/mmol) and [³H]methyl-4-phenylpyridinium ([³H]MPP⁺; 80 Ci/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO, USA) while [³H]dopamine (28 Ci/mmol) and [³H]5-HT (38 Ci/mmol) were purchased from Perkin Elmer (Billerica, MA, USA). All other reagents used for monoamine transporter assays were obtained from Sigma-Aldrich unless otherwise indicated.