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Lactate detection kit

Manufactured by Merck Group
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The Lactate detection kit is a laboratory product manufactured by Merck Group. It is designed to detect and quantify the presence of lactate in various biological samples. The kit provides a reliable and accurate method for measuring lactate levels, which is a key biomarker for various physiological and clinical applications.

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Lab products found in correlation

Acetyl coa assay kit, by Merck Group (1 mentions) Elisa reader, by Tecan (1 mentions)

4 protocols using lactate detection kit

1

Lactate Quantification in Cancer Samples

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The supernatants from B16-F10 or Hepa1-6 cells with different treatments, and the plasma from tumor mice or cancer patients were collected. Then, the lactate levels were measured with a lactate detection kit from Sigma–Aldrich according to the manufacturer’s instructions.
Luo M., Zhu J., Ren J., Tong Y., Wang L., Ma S, & Wang J. (2022). Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis. Frontiers in Oncology, 12, 1036543.
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2

Metabolite Quantification in Cell Culture

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The amount of lactate in 100 μl cell culture supernatants was analyzed with a lactate detection kit (Sigma) according to the manufacturer’s instructions in a 96 well plate format. The deproteinated samples were measured at 570 nm in an ELISA reader (Tecan) after enzymatic reaction. Three independent experiments were performed using three technical replicates in each run and the results were expressed as mean ± SD using bar charts.
For the detection of pyruvate and citrate in cell lysates commercial kits (Biovision) were used following the manufacturer’s protocol. Samples and standards were measured with an ELISA reader as described above.
Acetyl-CoA was measured fluorimetrically with an acetyl-CoA assay kit following the manufacturer’s instructions (Sigma).
The amount of glucose in the culture medium was controlled with the DNSA method as described by Miller [66 ].
Steven A., Leisz S., Wickenhauser C., Schulz K., Mougiakakos D., Kiessling R., Denkert C, & Seliger B. (2017). Linking CREB function with altered metabolism in murine fibroblast-based model cell lines. Oncotarget, 8(57), 97439-97463.
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3

Lactate Quantification in H2O2-Treated NP Cells

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First, the NP cells were stimulated with different concentrations of H2O2 for 30 min, after which the cells were washed three times using DMEM/F12 medium and incubated in a cell incubator for 24 h. According to the manufacturer's instructions for the lactate detection kit (Sigma-Aldrich, lot:7G16K06070), a 1-nmol/μL lactate standard solution was prepared and used to plot the standard curve. The solution was then centrifuged and deproteinated using a 10-kDa MWCO spin filter to remove lactate dehydrogenase. The lactate concentration was measured using a colorimetric assay. The pH of the sample solution was determined using a pH microelectrode meter.
Li Z., Cai F., Tang J., Xu Y., Guo K., Xu Z., Feng Y., Xi K., Gu Y, & Chen L. (2022). Oxygen metabolism-balanced engineered hydrogel microspheres promote the regeneration of the nucleus pulposus by inhibiting acid-sensitive complexes. Bioactive Materials, 24, 346-360.
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4

Measuring Lactate and Extracellular pH

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Lactate concentrations were measured using the lactate detection kit (Sigma) according to the manufacturer's instructions using supernatants of VHL− and VHL+ cells incubated for 48 h under normoxic and hypoxic conditions. In parallel, the extracellular pH was determined in the same supernatants used using a digital pH meter (p525, WTW). All experiments were carried out in three independent assays and the results are represented as the mean value of assays.
Leisz S., Schulz K., Erb S., Oefner P., Dettmer K., Mougiakakos D., Wang E., Marincola F.M., Stehle F, & Seliger B. (2015). Distinct von Hippel-Lindau gene and hypoxia-regulated alterations in gene and protein expression patterns of renal cell carcinoma and their effects on metabolism. Oncotarget, 6(13), 11395-11406.
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