Signa clinical scanners

Manufactured by GE Healthcare

Sourced in United States

The Signa clinical scanners are a range of magnetic resonance imaging (MRI) systems developed by GE Healthcare. These scanners utilize powerful magnetic fields and radio waves to generate detailed images of the body's internal structures, enabling healthcare professionals to diagnose and monitor a variety of medical conditions.

Automatically generated - may contain errors

Lab products found in correlation

Dmx 400 wb, by Bruker (4 mentions) Avance 2 nmr, by Bruker (4 mentions)

Close spelling variants of this product

Signa scanner Scanners

5 protocols using signa clinical scanners

Magnetic Labeling of Breast Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SK-BR-3, MCF-7, and the patients’ breast cancer cells were labeled with the super paramagnetic anti-HER-2 and anti-phosphatidylserine (anti-PS) antibodies propidium iodide, and bisbenzimide (40). The antibodies were dissolved and all washing steps carried in phenol-free, Ca⁺/Mg⁺ free, PIPES buffered saline solution, supplemented with 20 mM glucose, 10 % human serum. The aliquots were dispensed into the magnetism-free NMR tubes (Shigemi, Tokyo, Japan). The relaxation times T1 were measured in resonance to the applied pulse sequences on the NMR spectrometers: DMX 400 WB or AVANCE II NMR (Bruker, Billerica, MA) or the Signa clinical scanners (GE, Milwaukee, WI, USA).
The superparamagnetic antibodies were also used to isolate the labeled cells from the solution. The cancer cells labeled with the superparamagnetic antibodies were isolated on the magnetic, activated cell sorter operated at 1.5T (NSF grant support to MM).

Malecki M., Sabo C., Foorohar A, & Tombokan X. (2016). Novel paradigm for immunotherapy of breast cancer by engaging prophylactic immunity against hepatitis B. Clinical and Translational Medicine, 5, 32.

+ Open protocol

+ Expand

Magnetic Sorting of Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were labeled for positive selection with the superparamagnetic antibodies targeting TRA-1-60 and SSEA-4, and for the negative selection targeting CD45, CD34, dsDNA, and PS, while suspended in the physiological buffer supplemented with serum and glucose. The small aliquots were dispensed into the magnetism-free NMR tubes (Shigemi, Tokyo, Japan). The relaxation times T1 were measured in resonance to the applied FLAIR pulse sequences on the NMR spectrometers: DMX 400 WB or AVANCE II NMR (Bruker, Billerica, MA) or the Signa clinical scanners (GE, Milwaukee, WI, USA). The superparamagnetic antibodies were also used to isolate the labeled cells from the solution using the magnetic sorter to reach above 99.5% of purity (the sorter designed and built based upon the NSF funds for Dr Marek Malecki, Principal Investigator).

Malecki M., Putzer E., Sabo C., Foorohar A., Quach C., Stampe C., Beauchaine M., Tombokan X., Malecki R, & Anderson M. (2014). Directed cardiomyogenesis of autologous human induced pluripotent stem cells recruited to infarcted myocardium with bioengineered antibodies. Molecular and cellular therapies, 2, 13.

+ Open protocol

+ Expand

Magnetic Labeling and Isolation of Ovarian Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ovarian cancer cells were labeled with the superparamagnetic anti-HER-2 and anti-phosphatidylserine (anti-PS) antibodies [8 (link)]. The antibodies were dissolved and all washing steps carried in phenol-free, Ca+/Mg+—free, PIPES buffered saline solution, supplemented with 20 mM glucose, 10% human serum. The aliquots were dispensed into the magnetism-free NMR tubes (Shigemi, Tokyo, Japan). The relaxation times T1 were measured in resonance to the applied pulse sequences on the NMR spectrometers: DMX 400 WB or AVANCE II NMR (Bruker, Billerica, MA) or the Signa clinical scanners (GE, Milwaukee, WI, USA). The superparamagnetic antibodies were also used to isolate the labeled cells from the solution. The cells labeled with the superparamagnetic antibodies were isolated on the magnetic, activated cell sorter operated at 1.5 T (the NSF grant support to MM).

Malecki M., Putzer E., Quach C., Dodivenaka C, & Tombokan X. (2016). Novel paradigm for immunotherapy of ovarian cancer by engaging prophylactic immunity against hepatitis B virus. Clinical and Translational Medicine, 5, 44.

+ Open protocol

+ Expand

Magnetic Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were labeled for positive selection with the superparamagnetic antibodies targeting TRA-1-60, TRA-1-81, SSEA-3, and SSEA-4, and for the negative selection targeting CD45, CD34, dsDNA, and PS, while suspended in the physiological buffer supplemented with serum and glucose. The small aliquots were dispensed into the magnetism-free NMR tubes (Shigemi, Tokyo, Japan). The relaxation times T1 were measured in resonance to the applied FLAIR pulse sequences on the NMR spectrometers: DMX 400 WB or AVANCE II NMR (Bruker, Billerica, MA) or the Signa clinical scanners (GE, Milwaukee, WI, USA). The superparamagnetic antibodies were also used to isolate the labeled cells from the solution using the magnetic sorter to reach above 99.5% of purity (the sorter designed and built based upon the NSF funds for Dr M. Malecki, PI).

+ Open protocol

+ Expand

Superparamagnetic Biomolecule Isolation and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were labeled with the genomically engineered molecules (GEM)
rendered superparamagnetic, as described in details elsewhere. [25 (link)–26 (link),
29 –30 , 45 (link)–46 ] Briefly, the
AVA, VR, and VUV were dissolved and all washing steps carried in phenol-free,
Ca+ / Mg+- free, PIPES buffered saline solution, supplemented with 20 mM
glucose, 10% human serum. The aliquots were dispensed into the magnetism-free
NMR tubes (Shigemi). The relaxation times T1 and T2 were measured in resonance
to the applied pulse sequences on the NMR spectrometers: DMX 400 WB or AVANCE II
NMR (Bruker, Billerica, MA) or the Signa clinical scanners
(General Electric, Milwaukee, WI).
The superparamagnetic AVA, VER, and VUV were used to isolate the labeled
molecules and/or cells from the solution. The labeled cells rendered
superparamagnetic properties, which facilitated their isolation on the magnetic,
activated cell sorter (MACS) operated at 0.47 T – 9.4 T and / or clinical
MRI instruments operating at 1.5 T – 3 T and / or NMR scanners operating
at 0.47 T- 9.4 T (Bruker, Billerica, MA) (some results were validated thanks to
the access to the NSF and NIH instrumentation attained by MM).

Malecki M, & Saetre B. (2018). HIV Universal Vaccine. Molecular and cellular therapies, 6(1), 5.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!