Sugar analysis was performed as described previously [30 (link)]. The liquid fraction was neutralized to pH 5.0 by the addition of calcium hydroxide. An aliquot of sample (1.5 μL) was mixed with 1.5 μL of 0.1 % (w/w) Ribitol as internal standard, and then the mixture was dried in a vacuum concentrator (7810010; Labconco, Kansas City, MO, USA). The dried residue was dissolved in 100 μL of 20 mg/mL of methoxyamine hydrochloride in pyridine and incubated at 30 °C for 90 min, after which 50 μL of N-methyl-N-trimethylsilyltrifluoroacetamide was added and the sample was incubated at 37 °C for 30 min. A 10 μL aliquot of the solution was subjected to gas chromatography-mass spectrometry (GC–MS) (GCMS-2010plus; Shimadzu, Kyoto, Japan) under the following conditions: column, Agilent CP-Sil 8CB-MS (30 m × 0.25 mm); carrier gas, helium; injection temperature, 230 °C; oven temperature, 80 °C at t = 0–2 min, then increased to 330 °C at 15 °C/min.
Cp sil 8cb ms
Manufactured by Agilent Technologies
Sourced in Japan
The CP-Sil 8CB-MS is a capillary gas chromatography (GC) column designed for the separation and analysis of a wide range of organic compounds, including non-polar and slightly polar compounds. It features a 5% phenyl-95% dimethylpolysiloxane stationary phase, which provides enhanced separation and inertness for accurate and reproducible results.
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Lab products found in correlation
6 protocols using cp sil 8cb ms
1
Quantitative Sugar Analysis by GC-MS
2
GC-MS Analysis of Derivatized Sugars
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For sugar analysis, each sample (1.5 μL) was mixed with 1.5 μL of 0.1% (w/w) Ribitol, and the mixture was then dried in a vacuum concentrator (7810010; Labconco, Kansas City, MO, USA). The dried residue was dissolved in 100 μL of 20 mg/mL methoxyamine hydrochloride in pyridine and incubated at 30°C for 90 min, after which 50 μL of N-methyl-N-trimethylsilyltrifluoroacetamide was added and the sample was incubated at 37°C for 30 min. A 10-μL aliquot of the sample solution was subjected to gas chromatography-mass spectrometry (GC-MS) on a GC-MS-2010 plus system (Shimadzu, Kyoto, Japan) under the following conditions: column, Agilent CP-Sil 8CB-MS (30 m × 0.25 mm); carrier gas, helium; injection temperature, 230°C; oven temperature, 80°C at t = 0 to 2 min, then to 330°C at 15°C/min.
3
Byproduct Analysis of Liquid Fraction
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Byproduct analysis was performed as described previously [30 (link)]. Acetone (900 μL) was added to 100 µL of liquid fraction and mixed thoroughly. The sample was then centrifuged at 21,880×g and room temperature for 10 min. The supernatant (10 µL) was subjected to GC–MS analysis (GC-MS-2010plus; Shimadzu). 5-HMF and furfural were analyzed under the following conditions: column, Agilent CP-Sil 8CB-MS (30 m × 0.25 mm); carrier gas, helium; injection temperature, 250 °C; oven temperature, 50 °C at t = 0–5 min, then increased to 280 °C at 20 °C/min. Acetic acid and formic acid were analyzed under the following conditions: column, Agilent DB-FFAP (60 m × 0.25 mm); carrier gas, helium; injection temperature, 250 °C; oven temperature, 100 °C at t = 0 to 5 min, then increased to 230 °C at 10 °C/min.
4
GC-MS Analysis of 5-HMF and Furfural
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Acetone (900 μL) was added to 100 μL of liquid hydrolysate and mixed thoroughly, then the sample was centrifuged at 21,880 × g at room temperature for 10 min. An aliquot (10 μL) of the resulting supernatant was subjected to GC-MS analysis on a GC-MS-2010 plus instrument (Shimadzu). The following conditions were used for analysis of 5-HMF and furfural: column, Agilent CP-Sil 8CB-MS (30 m × 0.25 mm); carrier gas, helium; injection temperature, 250°C; oven temperature, 50°C at t = 0 to 5 min, then to 280°C at 20°C/min. Formate was analyzed under the following conditions: column, Agilent DB-FFAP (60 m × 0.25 mm); carrier gas, helium; injection temperature, 250°C; oven temperature, 100°C at t = 0 to 5 min, then to 230°C at 10°C/min.
5
Organic Acid Profiling in Urine
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Organic acids in urine were measured in a routine workflow of a clinical Biochemical Genetics laboratory (Department of Laboratory Medicine, Cliniques universitaires St Luc, UCLouvain, Brussels, Belgium). Briefly, urine was acidified by addition of hydrochloric acid and was extracted three times with ethyl acetate. The pooled organic phase was dried down under nitrogen flow, and organic acids were trimethylsilylated and analyzed by gas chromatography–mass spectrometry (ThermoScientific, Trace 1310) using an Agilent CP-Sil 8 CB-MS 30 m x 0.25 mm x 0.25 μm capillary column (43 ).
6
Comprehensive GC-MS Analysis of Extracts
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The composition of the extracts was investigated by GC-MS performed on a Varian Saturn 2200 GC system equipped with a capillary column with CP-Sil8CB-MS (30 m × 0.25 mm × 0.25 um) (Agilent Technologies,
www.chem.agilent.com ). The GC settings were as follows: the initial oven temperature was held at 50°C for 3 min and ramped at 20 °C/min to 120°C without reserve and then ramped at 10°C/min to 250°C for 5 min. The temperatures of the injector, interface, and gasification chamber were maintained at 260, 250, and 250°C, respectively. The samples (0.4 μL) were injected neat, with a split ratio of 1:100. The carrier gas was helium (99.999%). Mass spectra were obtained at 70 eV by electron impact ionization source. The temperatures of the interface and iron trap were 250 and 150°C. The electron multiplier voltage was 2.4 kV. The mass range analyzed was 20 amu to 650 amu. Most constituents were identified using GC by comparison of their retention indices based on literature data and MS data obtained from Saturn and NIST libraries. Component relative percentages were calculated based on GC peak areas without using correction factors (
Gholivand et al. 2009
;
Chu et al. 2011 (link)
).
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The composition of the extracts was investigated by GC-MS performed on a Varian Saturn 2200 GC system equipped with a capillary column with CP-Sil8CB-MS (30 m × 0.25 mm × 0.25 um) (Agilent Technologies,
Gholivand et al. 2009
;
Chu et al. 2011 (link)
).
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