Cd36 percpcy5

Manufactured by BD

CD36-PerCPCy5.5 is a fluorescent-labeled antibody used for the identification and quantification of CD36 expressing cells in flow cytometry applications. CD36 is a cell surface glycoprotein that functions as a receptor for various ligands. The PerCPCy5.5 fluorochrome is conjugated to the CD36 antibody, allowing for the detection and analysis of CD36 positive cells.

Automatically generated - may contain errors

Lab products found in correlation

Clinimacs, by Miltenyi Biotec (3 mentions) Cd34 bv421, by BioLegend (3 mentions) Cd135 pe, by BioLegend (3 mentions) Cd45ra bv711, by BioLegend (3 mentions) Cd38 pecf594, by BD (3 mentions) Lineage cocktail bv510, by BD (3 mentions) Cd110 apc, by BD (3 mentions) Cd41a apch7, by BD (3 mentions)

3 protocols using cd36 percpcy5

Isolation and Characterization of Hematopoietic Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34+ enriched (CliniMACS; Miltenyi), stained with Lineage cocktail-BV510 (BD), CD34-BV421 (Biolegend), CD38-PECF594(BD), CD45Ra-BV711, CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD) and CD41a-APCH7(BD) antibodies. Human MEP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻-CD41a⁻), ErP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^highCD110⁻), MkP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻CD41a⁺) and CMP (Lin⁻CD34⁺CD45Ra⁻CD135⁺) were sorted on a FACSAria, as previously described (Sanada et al., 2016 (link)).

Xavier-Ferrucio J., Ricon L., Vieira K., Longhini A.L., Lazarini M., Bigarella C.L., Franchi G J.r., Krause D.S, & Saad S.T. (2017). Hematopoietic defects in response to reduced Arhgap21. Stem cell research, 26, 17-27.

+ Open protocol

+ Expand

Isolation and Characterization of Hematopoietic Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Isolation of Hematopoietic Progenitor Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34⁺ enriched (CliniMACS; Miltenyi) and stained with Lineage cocktail-BV510 (BD Biosciences [BD]), CD34-BV421 (Biolegend), CD38⁻ PECF594 (BD), CD45Ra-BV711 (Biolegend), CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD), and CD41a-APCH7 (BD) antibodies. Human MEPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁻), MkPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁺), and ErPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38hiCD110⁻) were sorted on a FACSAria, as previously described^{3 (link)}.

Scanlon V.M., Thompson E.N., Lawton B.R., Kochugaeva M., Ta K., Mayday M.Y., Xavier-Ferrucio J., Kang E., Eskow N.M., Lu Y.C., Kwon N., Laumas A., Cenci M., Lawrence K., Barden K., Larsuel S.T., Reed F.E., Peña-Carmona G., Ubbelohde A., Lee J.P., Boobalan S., Oppong Y., Anderson R., Maynard C., Sahirul K., Lajeune C., Ivathraya V., Addy T., Sanchez P., Holbrook C., Van Ho A.T., Duncan J.S., Blau H.M., Levchenko A, & Krause D.S. (2022). Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation. Scientific Reports, 12, 16218.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!