Log In Sign up
The largest database of trusted experimental protocols

Chip it express enzyme kit

Manufactured by Active Motif
Visit Supplier

The ChIP-IT Express Enzyme kit is a laboratory tool designed for chromatin immunoprecipitation (ChIP) experiments. The kit provides the necessary reagents and enzymes to facilitate the fragmentation and immunoprecipitation of chromatin samples, which is a critical step in the ChIP process.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

ChIP-IT Express kit ChIP-IT Express ChIP-IT kit ChIP kit

2 protocols using chip it express enzyme kit

1

Chromatin Immunoprecipitation of Mfn2 Promoter

Check if the same lab product or an alternative is used in the 5 most similar protocols
Early passage Prdm16−/− MEFs were transduced with 10 MOI retrovirus for 72h and fixed with 4% paraformaldehyde for 10min. Protein lysates were isolated and chromatin immunoprecipiation was carried out using the ChIP-IT Express Enzyme kit (Active Motif, Carlsbad, CA). Antibodies used for ChIP include anti-FLAG and anti-TF2D. Primer probes were designed to span regions of the Mfn2 promoter previously shown to regulate Mfn2 transcriptional activity (see Supplementary Table 3).39 Quantification of precipitated Mfn2 promoter regions were derived from the linear regression of serial dilutions of BM genomic DNA, normalized to input DNA concentration and quantifiable IgG detection was subtracted from sample values.
Luchsinger L.L., de Almeida M.J., Corrigan D.J., Mumau M, & Snoeck H.W. (2016). Mitofusin 2 maintains hematopoietic stem cells with extensive lymphoid potential. Nature, 529(7587), 528-531.
+ Open protocol
+ Expand
2

Chromatin Immunoprecipitation of Mfn2 Promoter

Check if the same lab product or an alternative is used in the 5 most similar protocols
Early passage Prdm16−/− MEFs were transduced with 10 MOI retrovirus for 72h and fixed with 4% paraformaldehyde for 10min. Protein lysates were isolated and chromatin immunoprecipiation was carried out using the ChIP-IT Express Enzyme kit (Active Motif, Carlsbad, CA). Antibodies used for ChIP include anti-FLAG and anti-TF2D. Primer probes were designed to span regions of the Mfn2 promoter previously shown to regulate Mfn2 transcriptional activity (see Supplementary Table 3).39 Quantification of precipitated Mfn2 promoter regions were derived from the linear regression of serial dilutions of BM genomic DNA, normalized to input DNA concentration and quantifiable IgG detection was subtracted from sample values.
Luchsinger L.L., de Almeida M.J., Corrigan D.J., Mumau M, & Snoeck H.W. (2016). Mitofusin 2 maintains hematopoietic stem cells with extensive lymphoid potential. Nature, 529(7587), 528-531.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!