Porcine insulin ria kit

Body composition was determined from isotope dilution of ²H₂O at
the start of the study⁽²³⁾. For hormone analyses, commercially available kits were used. All
assays had previously been validated for use in cats; the assay procedures were performed
according to the manufacturers' instructions. Insulin was assayed in duplicate using the
porcine insulin RIA kit (catalogue no. PI-12 K-85 K; Linco Research,
Inc.)⁽²³⁾. Amylin was assayed in duplicate with a RIA kit specifically developed
for cats (catalogue no. RK-017-01; Phoenix Pharmaceuticals, Inc.)⁽²³⁾. Plasma ghrelin levels were measured in duplicate with a human active
ghrelin RIA kit from Linco Research (catalogue no. GHRA-88HK) that shows significant
cross-reactivity with feline ghrelin⁽²³⁾. PRL was assayed in triplicate as previously described in
cats⁽²⁶⁾.

Total cholesterol, high-density lipoprotein cholesterol (HDL-cholesterol), low-density lipoprotein cholesterol (LDL-cholesterol), triacylglycerols (TAG), phospholipids, total protein, urea, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) were analysed through diagnostic kits (Roche Diagnostics, Mannheim, Germany), using a Modular Hitachi Analytical System (Roche Diagnostics). Very low-density lipoprotein cholesterol (VLDL-cholesterol) and total lipids were calculated by Friedewald et al. [54 (link)] and Covaci et al. [55 ] formulas, respectively. Insulin and leptin concentrations were determined through the Porcine Insulin RIA kit (PI-12 K, Linco Research, Millipore, MA, USA) and the Multi-Species Leptin RIA kit (XL-85 K, Linco Research), respectively. To calculate the degree of insulin resistance, it was used the homeostasis model assessment using the insulin resistance index (HOMA-IR): fasting plasma glucose (mmol/L) times fasting plasma insulin (mU/L) divided by 22.5 [56 (link)]. Low HOMA-IR values indicate high insulin sensitivity, while high HOMA-IR values indicate high insulin resistance [56 (link)].

The plasma insulin concentration was determined using a commercial RIA kit (Porcine Insulin RIA kit; cat no PI-12K; Linco Research, St. Louis, MO, USA). The inter-assay coefficient of variation was 8.7%. The sensitivity of the assay was 1.611 μU/mL. The same kit for the sheep blood analysis was used by Catunda et al. [65 (link)].