Following preincubation for 10 min with an FT-GO reaction mixture that contained 10 µM FT, 3 µg/mL glucose oxidase (16831-14, Nacalai Tesque) and 1 or 2% bovine serum albumin in 0.1 M PB, FT-GO reaction was initiated by adding β-d -glucose (16804-32, Nacalai Tesque) into the reaction mixture at 20 µg/mL and proceeded for 30 min. The concentrations of glucose oxidase and β-d -glucose were determined in reference to our previous studies in BT-GO10 (link),11 (link). The FT used were: CF488A tyramide (92171, Biotium), CF568 tyramide (92173, Biotium) and CF647 tyramide (96022, Biotium).
Glucose oxidase
Manufactured by Nacalai Tesque
Sourced in Japan
Glucose oxidase is an enzyme that catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. It is commonly used in various laboratory applications, such as glucose analysis and biosensors.
Automatically generated - may contain errors
Lab products found in correlation
Diethyl ether, by Fujifilm (1 mentions)
Trisodium citrate dihydrate, by Fujifilm (1 mentions)
Uracil, by Fujifilm (1 mentions)
Myoglobin, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Fujifilm (1 mentions)
Potassium persulfate, by Fujifilm (1 mentions)
Bovine serum albumin (bsa), by Fujifilm (1 mentions)
Folin ciocalteu reagent, by Fujifilm (1 mentions)
Copper 2 sulfate pentahydrate, by Fujifilm (1 mentions)
2 protocols using glucose oxidase
1
Fluorescent Tyramide Labeling Optimization
2
Antioxidant Properties of Krill Extract
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Isada krill frozen and packed in a 1-kg plastic shrink wrap was purchased from Hamaichi (Wakayama, Japan) and stored in a freezer at -30°C until use. The average mass and length of the krill were 0.07±0.01 g and 18.00±0.65 mm, respectively. Diethyl ether, Folin-Ciocalteu reagent, trisodium citrate dihydrate, copper (II) sulfate pentahydrate, bovine serum albumin (BSA), 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothaiazoline-6-sulfuric acid) (ABTS), 6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (Trolox), and potassium persulfate, and uracil were purchased from Wako Pure Chemical Industries (Osaka, Japan). Myoglobin, tri -tyrosine, di-L-phenylalanine, and L-phenylalanyl-L-alanine dihydrate were purchased from Sigma-Aldrich (Tokyo, Japan). Glucose oxidase was purchased from Nacalai Tesque (Kyoto, Japan). Other reagents were of analytical grade.
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