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Elisa set for mouse il 6 and tnf α

Manufactured by BD

The ELISA set for mouse IL-6 and TNF-α is a laboratory equipment designed to measure the levels of these cytokines in mouse samples. It provides a quantitative assessment of the concentrations of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the tested samples.

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2 protocols using elisa set for mouse il 6 and tnf α

1

Quantifying Cytokines in Cell Culture Supernatant and Animal Samples

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Cytokines in supernatant from in vitro cultured cells or cytokines in the peritoneal lavage fluids, serum or lung homogenates from animal experiments were quantified using the ELISA set for mouse IL-6 and TNF-α (BD Biosciences) according to the manufacturer’s protocol.
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2

Quantification of Cytokine Levels

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Cytokines generated by in vitro cultured BMMs, peritoneal macrophages and MEFs were quantified using the ELISA Set for mouse IL-6 and TNF-α (BD Biosciences) according to the manufacturer’s protocol. ELISA for mouse IFN-β in cell culture supernatants and serum were performed using rat monoclonal anti-mouse IFN-β (Cosmo Bio USA #7891) as capture antibody and rabbit polyclonal anti-mouse IFN-β (R&D Systems #32400–1) as detection antibody, as previously described (Rothenfusser et al., 2005 (link)).
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