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2 protocols using calcium chloride

1

Osteogenic Differentiation of MC3T3-E1 Cells

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Calcium chloride (CaCl2), sodium dihydrogen phosphate (NaH2PO4), sodium hydroxide (NaOH), oleic acid, anhydrous ethanol and phosphate buffer (PBS) were obtained from Chengdu Kelong Chemical Co., Ltd. (Chengdu, Sichuan, China). Gelatin, methacrylic anhydride, ammonium persulfate (APS) and tetramethylethylenediamine (TEMED) were purchased from Aladdin Reagent Company (Shanghai, China). Fetal bovine serum (FBS) and α-Minimum Essential Medium (α-MEM) were purchased from Gibco (Grand Island, NY, USA). Streptomycin, penicillin and trypsin were purchased from Biosharp (Anhui, China). Dexamethasone, ascorbic acid-2 phosphate and β-glycerophosphate were purchased from Beijing Solarbio Sciences Co. (Beijing, China). Mouse calvaria-derived (subclone 14) osteoblast-like cells (MC3T3-E1) were purchased from Cobioer Co. (Nanjing, Jiangsu, China). The Sprague-Dawley rats used in this study were purchased from Chongqing Medical University Animal Care and Use Committee. All reagents were analytically pure and used as received. The experimental water was deionized water.
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2

Ziyan Tea Leaves Stability Analysis

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The experimental material was Ziyan tea fresh leaves from Mingshan District, Ya’an City, Sichuan Province, and the material tested was one bud and three leaves. Stability test materials, mainly including sodium chloride, potassium chloride, calcium chloride, magnesium chloride, aluminum chloride, zinc sulfate, copper sulfate, and sodium acetate were purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Wall materials mainly included maltodextrin, gum arabic, and soybean isolate protein, and were purchased from Xilong Chemical Co., Ltd. (Chengdu, China). All other chemicals were of analytical purity and purchased from Chengdu Kelong Reagent Factory. Distilled water was used throughout the experiments.
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