Aliquots of frozen blood from EDTA tubes were thawed and used to determine the level of MxA protein using the MxA Protein Human ELISA kit (BioVendor R&D) according to manufacturer's instructions. For cytokine analysis, plasma samples were analyzed using the LEGENDplex Human Anti‐Virus Response Panel multiplex assay (BioLegend, Inc.) according to the manufacturer's instructions. Samples were acquired on the Cytek Northern Lights 3000, and data were analyzed using BioLegend LEGENDplex Data Analysis Software.
Legendplex human anti virus response panel multiplex assay
Manufactured by BioLegend
Sourced in United States
The LEGENDplex Human Anti-Virus Response Panel is a multiplex assay designed to measure the levels of multiple human cytokines and chemokines associated with antiviral immune responses. It is a convenient tool for researchers studying host immune responses to viral infections.
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3 protocols using legendplex human anti virus response panel multiplex assay
1
ELISA and Multiplex Assay for MxA Protein and Cytokines
2
Quantification of MxA Protein and Cytokines
3
Multiplex Virus Response Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were seeded in 2 ml cell culture medium and supernatant was collected after 66 h, centrifuged at 300 x g, 4 °C for 5 min. and frozen in liquid nitrogen. Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol. The analysis was performed with the BD FACSVerse™ flow cytometer (BD Biosciences, San Jose, CA, USA). At least 300 events were acquired per analyte. Data were analyzed with the LEGENDplex v.8 software (BioLegend).
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