Dorsomorphin dihydrochloride, SB431542, CHIR99021 and Purmorphamine were purchased from Stemgent. Y27632, PD 0332991 isethionate and SU9516 were purchased from Tocris. Recombinant human GDNF, BDNF, NGF, bFGF and TGF-β3 were purchased from PerproTech. cAMP, Ara-C, Ara-A, ascorbic acid and N-Acetyl-L-cysteine were purchased from Sigma. FUW-tetO-LoxP-hOCT4, shP53pLKO.1, pMD2.G and psPAX2 were purchased from Addgene. Human Ascl1 (Genebank accession BC031299), Foxa2 (BC011780), Lmx1b (BC113491), FEV (BC023511.2), Nurr1 (CV028069), Lmx1a (BC06635), NeuroD1 (NM_002500), NeuroD2 (NM_006160) and Pitx3(NM_005029) were purchased from Open Biosystems and subcloned by PCR to the EcoRI site on the FUW-tetO-LoxP vector. Human miR-124 (MIMAT0000422) and Nkx2.2 (NM_002509) were amplified from normal human fibroblast genomic DNA and subcloned to the EcoRI site on the FUW-tetO-LoxP vector. FUW-LoxP-M2rtTA was generated by subcloning the BspEI fragment containing the loxP site from FUW-tetO-LoxP-hOCT4 (Addgene) to the BspEI site on the 3’LTR of FUW-M2rtTA (Addgene). All constructs were verified by sequencing.
Pitx3
Manufactured by Thermo Fisher Scientific
Sourced in United States
Pitx3 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a multipurpose tool used for various research and analytical applications. The core function of Pitx3 is to facilitate specific tasks and procedures within scientific laboratories, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Fuw m2rtta, by Addgene (2 mentions)
Ara a, by Merck Group (2 mentions)
Su9516, by Bio-Techne (2 mentions)
Pd 0332991 isethionate, by Bio-Techne (2 mentions)
Fuw teto loxp hoct4, by Addgene (2 mentions)
Shp53plko 1, by Addgene (2 mentions)
Human ascl1, by Thermo Fisher Scientific (2 mentions)
Ara c, by Merck Group (2 mentions)
Y 27632, by Bio-Techne (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
N acetyl l cysteine, by Merck Group (2 mentions)
Pspax2, by Addgene (2 mentions)
Pmd2 g, by Addgene (2 mentions)
2 protocols using pitx3
1
Generation of Induced Neuronal Cells
2
Induced Neurogenesis: Protocols and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dorsomorphin dihydrochloride, SB431542, CHIR99021 and Purmorphamine were purchased from Stemgent (Cambridge, MA, USA). Y27632, PD 0332991 isethionate and SU9516 were purchased from Tocris (Bristol, UK). Recombinant human GDNF, BDNF, NGF, bFGF and TGF-β3 were purchased from PerproTech (Rocky Hill, NJ, USA). cAMP, Ara-C, Ara-A, ascorbic acid and N-acetyl-L -cysteine were purchased from Sigma (St. Louis, MO, USA). FUW-tetO-LoxP-hOCT4, shP53pLKO.1, pMD2.G and psPAX2 were purchased from Addgene (Cambridge, MA, USA). Human Ascl1 (Genebank accession BC031299), Foxa2 (BC011780), Lmx1b (BC113491), FEV (BC023511.2), Nurr1 (CV028069), Lmx1a (BC06635), NeuroD1 (NM_002500), NeuroD2 (NM_006160) and Pitx3(NM_005029) were purchased from Open Biosystems (Pittsburgh, PA, USA) and subcloned by PCR to the EcoRI site on the FUW-tetO-LoxP vector. Human miR-124 (MIMAT0000422) and Nkx2.2 (NM_002509) were amplified from normal human fibroblast genomic DNA and subcloned to the EcoRI site on the FUW-tetO-LoxP vector. FUW-LoxP-M2rtTA was generated by subcloning the BspEI fragment containing the loxP site from FUW-tetO-LoxP-hOCT4 (Addgene) to the BspEI site on the 3′LTR of FUW-M2rtTA (Addgene). All constructs were verified by sequencing.
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