Primary cardiomyocytes were separated and maintained in DMEM containing 4.5 g/L of glucose and supplemented with 10% (v/v) FBS, and 1% penicillin/streptomycin at 37°C in a humidified 5% CO2 incubator. The cardiomyocytes were pretreated with 2.5, 5, and 10 µM emodin (E106693; Aladdin, Shanghai, China), 5 µM Bay-117082 (NF-κB pathway inhibitor) (S2913; Selleck, Shanghai, China), 10 µM NLRP3 inflammasome inhibitor (S3680; Selleck), or N-acetylcysteine (NAC, 1 mM, S0077; Beyotime, Shanghai, China) for 1 hour before the cardiomyocytes were stimulated with hypoxia/reoxygenation (H/R).
S3680
Manufactured by Selleck Chemicals
Sourced in China
The S3680 is a versatile laboratory equipment used for general scientific applications. It serves as a reliable tool for researchers and laboratory professionals. The core function of the S3680 is to perform standard laboratory tasks with precision and efficiency.
Automatically generated - may contain errors
2 protocols using s3680
1
Cardiomyocyte hypoxia/reoxygenation model
2
Agmatine Modulates Microglial Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mouse microglial cell line, BV2, was purchased from the German Collection of Microorganisms and cell cultures (DSMZ). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Waltham, MA, United States) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific) at 37°C in 5% CO2. Preliminary experiments were performed to determine the dose of agmatine. BV2 microglial cells were pretreated with 1, 10, 50, 100, 200 or 500 μM agmatine. The dose chosen for subsequent experiments was 100 μM agmatine.5 µM Bay-117082 (an NF-κB pathway inhibitor) (S2913; Selleck, Shanghai, China), or a Nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome inhibitor (10 µM, S3680; Selleck) was used to BV2 microglial cells for 1 h before LPS stimulation.
BV2 microglial cells were divided into four groups: the control group was administered sterile water; the AGM group was administered agmatine alone (100 μM); the LPS group was administered LPS (1 μg/ml) (Nam et al., 2018 (link)); and the LPS + AGM group was administered agmatine (100 μM) followed by LPS (1 μg/ml).
BV2 microglial cells were divided into four groups: the control group was administered sterile water; the AGM group was administered agmatine alone (100 μM); the LPS group was administered LPS (1 μg/ml) (Nam et al., 2018 (link)); and the LPS + AGM group was administered agmatine (100 μM) followed by LPS (1 μg/ml).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!