Anti rabbit

All antibodies were diluted in PBS with 1% skimmed milk. Human monoclonal antibodies against SARS-CoV-2 S (S2 subunit) were kindly provided by Florian Klein, University of Cologne (Köln, Germany) (HbnC5t1p1_B10, HbnC5t1p1_E5). We stained cis-Golgi compartments with a monoclonal mouse anti-GM130 antibody (BD Bioscience, #610822, dilution 1:5) and ERGIC with a monoclonal mouse anti-LMAN1 antibody (Invitrogen, #PA1-074, dilution 1:50). Myc-tagged Rab1A and Rab1B proteins were stained with a rabbit anti-Myc antibody (Cell Signaling, #2272S, dilution 1:50 for immunofluorescence assays (IFAs) and 1:250 for Western blot). Endogenous tubulin was stained with a monoclonal antibody from Sigma-Aldrich (#T9026, dilution 1:500). Alexa Fluor-labeled secondary antibodies were purchased from Invitrogen (Anti-human-Alexa Fluor 488, #A-11013; anti-mouse-Alexa Fluor 568, #A-11004; anti-mouse-Alexa Fluor 594, #A-11032; anti-rabbit-Alexa Fluor 594, #A-11012; anti-rabbit-Alexa Fluor 647, #A27040, secondary antibodies were diluted 1:400). For Western blot analyses, following HRP-coupled secondary antibodies were used: Anti-human (Invitrogen, Waltham, MA, USA, #PA1-28587, dilution 1:3000), anti-rabbit (Dianova, Geneva, Switzerland, #711-036-152, dilution 1:40,000) and anti-mouse (Dako, Glostrup, Denmark, #P044701-2 dilution 1:40,000).