All microscopy images were taken using the LSM 700 (Carl Zeiss) confocal microscope, LCI Plan-Neofluar 63x/1.30 glycerol immersion objective, at room temperature, and Zen Black 2.1 acquisition software (version 11.0.0.190, Carl Zeiss AG, Oberkochen, Germany). Image analysis was done using the Zen Blue Lite (version 2.0, Carl Zeiss AG, Oberkochen, Germany), PHOTO-PAINT, and CorelDraw programs from the CorelDRAW Graphics Suite 2017. Post-imaging processing was done using Corel PHOTO-PAINT 2017 (Corel Corporation, Ottawa, ON, Canada) using the brightness/contrast/intensity adjustment settings equally and simultaneously for images from the same imaging series.
Photo paint 2017
Manufactured by Corel
Sourced in United States
Corel PHOTO-PAINT 2017 is a professional image editing software developed by Corel Corporation. It provides a range of tools and features for manipulating and enhancing digital images. The software's core function is to allow users to edit, retouch, and composite images with precision and control.
Automatically generated - may contain errors
5 protocols using photo paint 2017
1
Confocal Imaging and Post-Processing
2
External Palp Morphology Imaging Protocol
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All overview images of the external palp morphology were taken using the Visionary Digital Imaging system. All image adjustments were carried out using either Adobe Photoshop CS6 (Adobe systems, Inc., San José, California, USA) or CorelDRAW 2017, Corel PHOTO-PAINT 2017 and Corel PaintShop Pro 2018 (all Corel Corp., Ottawa, Ontario, Canada).
The terminology for the description of the nervous tissue is based on the neuroanatomical glossary by Richter et al. [29 (link)]. Spider-specific terminology is based on the Spider Anatomy Ontology (SPD) [43 ].
The terminology for the description of the nervous tissue is based on the neuroanatomical glossary by Richter et al. [29 (link)]. Spider-specific terminology is based on the Spider Anatomy Ontology (SPD) [43 ].
3
Image Enhancing and Composing Protocols
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If necessary, the contrast of images was enhanced using Corel PHOTO-PAINT 2017. The plates were composed with CorelDRAW 2017 (both Corel Corp., Ottawa, Ontario, Canada).
4
Genital Morphology and Mating Status
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External genitalia of one virgin early-subadult female, one virgin and one mated late-subadult female and one virgin and one mated adult female were fixed in 70% ethanol and photographed using a customised Visionary Digital BK Plus imaging system (Dun, Inc., Palmyra, Virginia, USA). All image adjustments were carried out using either Adobe Photoshop CS6 and Illustrator CS4 (Adobe systems, Inc., San José, California, USA) or CorelDRAW 2017 and Corel PHOTO-PAINT 2017 (all Corel Corp., Ottawa, Ontario, Canada). Image stacking was processed using “Zerene Stacker” stacking software (Zerene Systems LLC, Richland, Washington, USA).
We inspected cuticles shed by virgin (N = 15) and mated (N = 15) subadult females during their final moult for a presence of spermathecae under a stereomicroscope (Carl Zeiss stereomicroscope 475,110–9904).
We inspected cuticles shed by virgin (N = 15) and mated (N = 15) subadult females during their final moult for a presence of spermathecae under a stereomicroscope (Carl Zeiss stereomicroscope 475,110–9904).
5
Micro-CT Data Processing and Visualization
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Processing, analysis and surface reconstructions of micro-computed tomography data were carried out using Amira 6.4 (FEI Software, now Thermo Fisher Scientific, Waltham, MA, USA). All overview photographs of the specimen were taken using a customized Visionary Digital BK Plus imaging system (Dun, Inc., Palmyra, VA, USA), either in ethanol or in the air (dried samples). All image adjustments were done using either Adobe Photoshop CS6 (Adobe Systems, Inc., San José, CA, USA) or CorelDRAW 2017, Corel PHOTOPAINT 2017, and Corel PaintShop Pro 2021 (all Corel Corp., Ottawa, Ontario, Canada).
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