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G2 spirit biotwin transmission

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The G2 Spirit BioTWIN transmission is a laboratory equipment product from Thermo Fisher Scientific. It is designed to provide power transmission for various laboratory applications. The core function of this product is to efficiently transfer mechanical power between components in a laboratory setting.

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Lab products found in correlation

Eagle 4k hs digital camera, by Thermo Fisher Scientific (4 mentions) Uct ultramicrotome, by Leica (3 mentions) Durcupan epoxy resin, by Merck Group (2 mentions)

5 protocols using g2 spirit biotwin transmission

1

Ultrastructural Analysis of Adrenal Medulla

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The mice were euthanized with isoflurane and perfused through the heart with modified Karnovsky’s fixative solution (2% paraformaldehyde and 2.5% glutaraldehyde in 0.15mol/l sodium cacodylate buffer, pH 7.4) and then the adrenal glands were dissected. Glands were kept in 2% OsO4 in 0.15 mol/l sodium cacodylate buffer followed by 2% uranyl acetate and subsequently subjected to dehydration with graded series of alcohol and infiltration with Durcupan epoxy resin. Ultrathin sections were cut at 60 nm and mounted on 300 mesh copper grids. Sections were stained with 2% uranyl acetate and Sato’s lead stain for better contrast [26 (link)]. Grids were viewed using a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera (FEI, Hilsboro, Oregon, USA) for imaging. At least four animals were used for each cohort and 20 micrographs from each mouse were examined for the ultrastructure of adrenal medulla. DCV were analyzed for their abundance, granule size and granule percentage area.
Mir S.A., Li Y., Story J.D., Bal S., Awdishu L., Street A.A., Mehta R.L., Singh P, & Vaingankar S.M. (2018). Mice overexpressing chromogranin A display hypergranulogenic adrenal glands with attenuated ATP levels contributing to the hypertensive phenotype. Journal of hypertension, 36(5), 1115-1128.
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2

Ultrastructural Imaging of Cellular Organelles

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Cell pellets were immersed in modified Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 0.15 M sodium cacodylate buffer, pH 7.4), post-fixed in 1% osmium tetroxide in 0.15 M cacodylate buffer and stained en bloc in 2% uranyl acetate for. Samples were dehydrated in ethanol, embedded in Durcupan epoxy resin, sectioned at 50 to 60 nm on a Leica UCT ultramicrotome, and picked up on Formvar and carbon-coated copper grids. Sections were stained with 2% uranyl acetate for 5 minutes and Sato’s lead stain for 1 minute. Images were taken on a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera using a software TIA (FEI, Hillsboro, OR) at University of California, San Diego, Cellular and Molecular Medicine Electron Microscopy Facility.
Ishii Y., Nhiayi M.K., Tse E., Cheng J., Massimino M., Durden D.L., Vigneri P, & Wang J.Y. (2015). Knockout Serum Replacement Promotes Cell Survival by Preventing BIM from Inducing Mitochondrial Cytochrome C Release. PLoS ONE, 10(10), e0140585.
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3

Transmission Electron Microscopy Specimen Preparation

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Samples were immersed in modified Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 0.15 M sodium cacodylate buffer, pH 7.4) for at least 4 hours, postfixed in 1% osmium tetroxide in 0.15 M cacodylate buffer for 1 hour and stained en bloc in 2% uranyl acetate for 1 hour. Samples were dehydrated in ethanol, embedded in Durcupan epoxy resin (Sigma-Aldrich), sectioned at 50 to 60 nm on a Leica UCT ultramicrotome, and picked up on Formvar and carbon-coated copper grids. Sections were stained with 2% uranyl acetate for 5 min and Sato's lead stain for 1 min. Grids were viewed using a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera (FEI).
Akizu N., Cantagrel V., Zaki M.S., Al-Gazali L., Wang X., Rosti R.O., Dikoglu E., Gelot A.B., Rosti B., Vaux K.K., Scott E.M., Silhavy J.L., Schroth J., Copeland B., Schaffer A.E., Gordts P., Esko J.D., Buschman M.D., Fields S.J., Napolitano G., Ozgul R.K., Sagiroglu M.S., Azam M., Ismail S., Aglan M., Selim L., Gamal I., Hadi S.A., El Badawy A., Sadek A.A., Mojahedi F., Kayserili H., Masri A., Bastaki L., Temtamy S., Müller U., Desguerre I., Casanova J.L., Dursun A., Gunel M., Gabriel S.B., de Lonlay P, & Gleeson J.G. (2015). Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction. Nature genetics, 47(5), 528-534.
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4

Transmission Electron Microscopy Specimen Preparation

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Samples were immersed in modified Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 0.15 M sodium cacodylate buffer, pH 7.4) for at least 4 hours, postfixed in 1% osmium tetroxide in 0.15 M cacodylate buffer for 1 hour and stained en bloc in 2% uranyl acetate for 1 hour. Samples were dehydrated in ethanol, embedded in Durcupan epoxy resin (Sigma-Aldrich), sectioned at 50 to 60 nm on a Leica UCT ultramicrotome, and picked up on Formvar and carbon-coated copper grids. Sections were stained with 2% uranyl acetate for 5 min and Sato's lead stain for 1 min. Grids were viewed using a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera (FEI).
Akizu N., Cantagrel V., Zaki M.S., Al-Gazali L., Wang X., Rosti R.O., Dikoglu E., Gelot A.B., Rosti B., Vaux K.K., Scott E.M., Silhavy J.L., Schroth J., Copeland B., Schaffer A.E., Gordts P., Esko J.D., Buschman M.D., Fields S.J., Napolitano G., Ozgul R.K., Sagiroglu M.S., Azam M., Ismail S., Aglan M., Selim L., Gamal I., Hadi S.A., El Badawy A., Sadek A.A., Mojahedi F., Kayserili H., Masri A., Bastaki L., Temtamy S., Müller U., Desguerre I., Casanova J.L., Dursun A., Gunel M., Gabriel S.B., de Lonlay P, & Gleeson J.G. (2015). Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction. Nature genetics, 47(5), 528-534.
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5

Quantifying Bacterial Flagellation Phenotypes

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After growth, strains were resuspended from MH agar into PBS, pelleted for 3 min at 13 200 r.p.m. in a microcentrifuge, resuspended in 2% gluteraldehye, and then incubated on ice for 1 h. Samples were then stained with 2% uranyl acetate and visualized with an FEI Technai G2 Spirit Bio TWIN transmission electron microscope. Flagellar numbers were counted from at least 100 individual cells and averaged from two biological replicates to determine the proportion of bacterial populations producing different flagellation phenotypes: hyperflagellated (a bacterium producing at two or more flagella at least at one pole); wild-type, (producing a single flagellum at both poles or a flagellum at one pole with the other pole aflagellated); or aflagellated (lacking a flagellum). After averaging, the standard deviation for each population was calculated. The Student's t-test was used to evaluate statistical significance of the hyperflagellation phenotype in flhG mutants relative to WT FlhG.
Gulbronson C.J., Ribardo D.A., Balaban M., Knauer C., Bange G, & Hendrixson D.R. (2015). FlhG employs diverse intrinsic domains and influences FlhF GTPase activity to numerically regulate polar flagellar biogenesis in Campylobacter jejuni. Molecular microbiology, 99(2), 291-306.
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