Rat il 1β platinum elisa kit

For brain region homogenate, measurement of interleukin 1 beta (IL-1β; proinflammatory cytokine) was estimated using commercially available rat IL-1β ELISA Kit (Wuhan USCN Business Co., Ltd., Wuhan, China) following the manufacturer's protocol. For the serum, quantitative measurement of IL-1β was estimated using commercially available rat IL-1β platinum ELISA Kit (eBioscience, San Diego, USA) following the manufacturer's protocol.

Retro-orbital blood samples were withdrawn from thiopental (50 mg/kg i. p.) anesthetized rats prior to kidney isolation. The collected blood was permitted to coagulate for 15 min at room temperature then centrifuged at 1,200 g for 10 min. The aspirated serum was stored at −80°C for subsequent ELISA determination of IL-1β (Rat IL-1β Platinum ELISA Kit, eBioscience ™ BMS630, United States) and TNFα (Rat TNF alpha Platinum ELISA Kit, eBioscience ™ BMS622, United States) according to the manufacturer’s protocol. Creatinine was determined colorimetrically using commercially available kits (BioSystems, Spain).