Secondary goat anti mouse apc

For protein microarray analysis, 2 · 10⁵ HUVEC and HSaVEC were infected with RV at an MOI of ten in two independent experiments. Supernatants were collected 48 hpi and analyzed for the presence of chemokines using the Proteome Profiler Human Chemokine Array Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. For flow cytometry analysis, 1°10⁵ HUVEC and HSaVEC were infected with RV at an MOI of 5 and detached with trypsin at various time points post infection. Following fixation with 2 % paraformaldehyde for 20 min, cells were permeabilized with 0.5 % saponine and stained with a RV anti-capsid antibody (Meridian Life Science, USA) and a secondary goat anti-mouse APC (Biolegend, USA). Flow cytometry data were acquired on a FACSCalibur flow cytometer (BD Biosciences, USA) using Cell Quest Pro software (BD Biosciences).