For immunofluorescence microscopy, the following antibodies were used: monoclonal mouse anti-collagen type 5 (1 mg/ml) from Sigma-Aldrich (catalog no. sc-166155), monoclonal mouse anti-collagen type 5 Alexa Fluor 488 conjugate from Sigma-Aldrich (catalog no. sc-166-155 AF488), polyclonal rabbit anti–collagen type 1 from Rockland (catalog no. 600-401-103-0.5), monoclonal mouse anti–fibulin 1 from Santa Cruz Biotechnology (catalog no. sc-25281), monoclonal mouse anti–fibulin 1 Alexa Fluor 647 conjugate (1 mg/ml) from Santa Cruz Biotechnology (catalog no. sc-25281 AF647), and polyclonal rabbit antifibronectin (1 mg/ml) from Santa Cruz Biotechnology (catalog no. sc-9068). Hoechst-33342 was obtained from Sigma-Aldrich (catalog no. B2261). The following secondary antibodies were used: Alexa Fluor 488 donkey anti-mouse Ab (Invitrogen, catalog no. A21202), Alexa Fluor 568 donkey anti-mouse Ab (Invitrogen, catalog no. A11004), and Alexa Fluor 568 donkey anti-mouse Ab (Invitrogen, catalog no. A11011). For immunofluorescence stainings of actin stress fibers, Alexa Fluor 568 Phalloidin (Invitrogen, A12380) was used. 4′,6-Diamidino-2-phenylindole (DAPI) was acquired from Sigma-Aldrich (catalog no. D9564).
Gerckens M., Schorpp K., Pelizza F., Wögrath M., Reichau K., Ma H., Dworsky A.M., Sengupta A., Stoleriu M.G., Heinzelmann K., Merl-Pham J., Irmler M., Alsafadi H.N., Trenkenschuh E., Sarnova L., Jirouskova M., Frieß W., Hauck S.M., Beckers J., Kneidinger N., Behr J., Hilgendorff A., Hadian K., Lindner M., Königshoff M., Eickelberg O., Gregor M., Plettenburg O., Yildirim A.Ö, & Burgstaller G. (2021). Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics. Science Advances, 7(52), eabb3673.
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