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Endoproteinases glu c

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Endoproteinases Glu-C is a laboratory reagent used for protein analysis. It is an endoprotease enzyme that cleaves peptide bonds on the carboxyl side of glutamic acid residues in proteins.

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Lab products found in correlation

Pcr purification kit, by Qiagen (2 mentions) Taq ligase, by New England Biolabs (2 mentions) Lys c, by Roche (2 mentions) Gel extraction, by Qiagen (2 mentions) Electrocompetent e coli dh5α cells, by New England Biolabs (2 mentions) Dntp solutions, by New England Biolabs (2 mentions) Peptide calibration standard 2 for maldi tof ms, by Bruker (2 mentions) Pbad hisa vector, by Thermo Fisher Scientific (2 mentions) Phusion high fidelity dna polymerase, by New England Biolabs (2 mentions) T4 dna ligase, by New England Biolabs (2 mentions) Restriction endonuclease, by New England Biolabs (2 mentions) Oligonucleotide primers, by Integrated DNA Technologies (2 mentions) Protein calibration standard 1, by Bruker (2 mentions)

Close spelling variants of this product

Glu-C (27 citations) Sequencing grade endoproteinase Glu-C (2 citations)

2 protocols using endoproteinases glu c

1

Recombinant Protein Expression in E. coli

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All chemicals used were purchased from Sigma Aldrich or Fisher Scientific unless noted otherwise. Endoproteinases Glu-C and Lys-C were purchased from Roche Applied Science. Oligonucleotide primers used for molecular cloning were purchased from Integrated DNA Technologies. The pBAD/HisA vector was purchased from Invitrogen. Electrocompetent E. coli DH5α cells, Phusion High-Fidelity DNA polymerase, Taq ligase, dNTP solutions, T4 DNA ligase and all restriction endonucleases were purchased from New England Biolabs. Gel extraction, plasmid miniprep, and PCR purification kits were purchased from QIAGEN. Protein Calibration Standard I and Peptide Calibration Standard II for MALDI-TOF MS were purchased from Bruker. E. coli DH5α was used as host for cloning and plasmid propagation, and E. coli BL21 (DE3) was used as a host for overexpression.
Yang X., Lennard K.R., He C., Walker M.C., Ball A.T., Doigneaux C., Tavassoli A, & van der Donk W.A. (2018). A lanthipeptide library used to identify a protein–protein interaction inhibitor. Nature chemical biology, 14(4), 375-380.
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2

Recombinant Protein Expression in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals used were purchased from Sigma Aldrich or Fisher Scientific unless noted otherwise. Endoproteinases Glu-C and Lys-C were purchased from Roche Applied Science. Oligonucleotide primers used for molecular cloning were purchased from Integrated DNA Technologies. The pBAD/HisA vector was purchased from Invitrogen. Electrocompetent E. coli DH5α cells, Phusion High-Fidelity DNA polymerase, Taq ligase, dNTP solutions, T4 DNA ligase and all restriction endonucleases were purchased from New England Biolabs. Gel extraction, plasmid miniprep, and PCR purification kits were purchased from QIAGEN. Protein Calibration Standard I and Peptide Calibration Standard II for MALDI-TOF MS were purchased from Bruker. E. coli DH5α was used as host for cloning and plasmid propagation, and E. coli BL21 (DE3) was used as a host for overexpression.
Yang X., Lennard K.R., He C., Walker M.C., Ball A.T., Doigneaux C., Tavassoli A, & van der Donk W.A. (2018). A lanthipeptide library used to identify a protein–protein interaction inhibitor. Nature chemical biology, 14(4), 375-380.
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