Gridion x5

Manufactured by Oxford Nanopore Technologies

The GridION X5 is a benchtop device designed for high-throughput DNA and RNA sequencing. It utilizes Oxford Nanopore's proprietary nanopore technology to enable real-time, long-read sequencing. The GridION X5 can accommodate up to 5 flow cells simultaneously, allowing for parallel processing of multiple samples.

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Lab products found in correlation

Promethion, by Oxford Nanopore Technologies (2 mentions) Smarter pcr cdna synthesis kit, by Illumina (2 mentions) Direct cdna sequencing kit, by Oxford Nanopore Technologies (2 mentions) Ligation sequencing kit, by Oxford Nanopore Technologies (2 mentions) Novaseq platform, by Illumina (2 mentions)

3 protocols using gridion x5

Multi-platform Transcriptome Sequencing

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For Illumina sequencing, cDNA libraries (with three biological replicates) were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Beverly, MA, USA) following the manufacturer’s protocol. The libraries were sequenced on an Illumina NovaSeq platform, and paired-end reads were generated.
For PacBio sequencing, cDNA from the same RNA samples used for Illumina sequencing was synthesised using the SMARTer PCR cDNA Synthesis Kit. After PCR amplification, products were sequenced on the PacBio Sequel platform.
For ONT sequencing, cDNA-PCR libraries were built using the Ligation Sequencing Kit (SQK-LSK109) and sequenced on the Nanopore PromethION platform. Direct-cDNA libraries were built using the Direct cDNA Sequencing Kit (SQK-DCS108) and sequenced on the Nanopore GridION X5 platform.

Cui J., shen N., Lu Z., Xu G., Wang Y, & Jin B. (2020). Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome. Plant Methods, 16, 85.

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Multiplatform Transcriptome Sequencing

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For Illumina sequencing, cDNA libraries (with three biological replicates) were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Beverly, MA, USA) following the manufacturer's protocol. The libraries were sequenced on an Illumina NovaSeq platform, and paired-end reads were generated.
For PacBio sequencing, cDNA from the same RNA samples used for Illumina sequencing was synthesised using the SMARTer PCR cDNA Synthesis Kit. After PCR ampli cation, products were sequenced on the PacBio Sequel platform.
For ONT sequencing, cDNA-PCR libraries were built using the Ligation Sequencing Kit (SQK-LSK109) and sequenced on the Nanopore PromethION platform. Direct-cDNA libraries were built using the Direct cDNA Sequencing Kit (SQK-DCS108) and sequenced on the Nanopore GridION X5 platform.

Cui J., Shen N., Lu Z., Xu G., Wang Y., & Jin B. (2020). Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome.

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Whole genome sequencing of Actinidia cantoniensis

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Root, leaf, and stem tissues of 'JGC-3', a popular cultivar of A. cantoniensis, were collected from the Guang Zhou City (Tianhe District, 23˚9′5.6448′′ N, 113˚21′22.8996′′ E) in Guangdong Province, China. High-quality genomic DNA was extracted using a QIAGEN Genomic Kit. The integrity of the DNA was checked by 0.75% agarose gel electrophoresis. Nanodrop (OD260/280 = 1.8-2.0, OD260/230 = 2.0-2.2) and Qubit were used to detect the DNA purity and the quantity respectively. The genome of A. cantoniensis was sequenced using Nanopore GridION X5.

Xu S., Li F., Wu B., Mei Y., Wang J, & Wang J. (2022). Complete genome sequence and phylogenetic analysis of medicinal plant Abrus cantoniensis for evolutionary research and germplasm utilization. The plant genome, 15(3).

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