Rabbit anti human mtor

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-human mTOR is a primary antibody that specifically recognizes the mammalian target of rapamycin (mTOR) protein in human samples. mTOR is a serine/threonine protein kinase that plays a central role in regulating cell growth, proliferation, and metabolism.

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Close spelling variants of this product

Rabbit anti-human mTOR monoclonal antibody (2 citations) Rabbit anti-mTOR (52 citations) Anti-mTOR (326 citations)

3 protocols using rabbit anti human mtor

Anticancer effects of natural compounds

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Lycorine (purity >98%) was obtained from Nanjing Purun Tech Co. and cisplatin (purity >98.5%) was obtained from Dalian Meilun Biotech. Curcumin (purity >98%) was purchased from Shanghai Jianglai Biotech and non-small cell lung cancer cell line A549 was from Nanjing Lishizi Biotech (Nanjing, China). Fetal bovine serum was purchased from Hyclone (Logan City, UT). DMEM were from Shanghai Feili i Biotech (Shanghai, China). Cell culture flasks, cell culture dishes, and 96- and 6-well plates were purchased from Tianjin Ruishina biotech (Tianjin, China). MTT and trypsin was bought from Nanjing Zhongshan Biotech (Nanjing, China). Wortmannin was purchased from Selleck (Houston, TX). Protein extraction kit and BCA protein quantification kit were from Shijiazhuang Haisen Chemical (Shijiazhuang, China). Mouse anti-human β-actin (Item No. 3700), rabbit anti-human AMPK (Item No 5831), rabbit anti-human mTOR (Item No. 2983), rabbit anti-human S6K (Item No. 2708), rabbit anti-human LC3 (Item No. 3868), rabbit anti-human caspase3 (Item No. 9662), rabbit anti-human phospho AMPK (Item No. 5256), and rabbit anti-human S6K (Item No. 9234) antibodies were bought from Cell Signaling Technology (Danvers, MA). Goat anti-rabbit IgG-HRP (sc-2004) and goat anti-mouse IgG-HRP (sc-2005) secondary antibodies were from Santa Cruz Biotechnology (Dallas, TX).

Zeng H., Fu R., Yan L, & Huang J. (2017). Lycorine Induces Apoptosis of A549 Cells via AMPK-Mammalian Target of Rapamycin (mTOR)-S6K Signaling Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 2035-2041.

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Protein Expression Analysis in Cell Lines

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Protein lysates from UM-HMC cells and dissociated xenograft MEC tissues were prepared using 1% Nonidet P-40 (NP-40) lysis buffer and loaded onto 9–12% SDS-PAGE gels. Membranes were incubated overnight at 4 °C with 1:1000 dilution of the following antibodies: rabbit antihuman p-AKT Ser 473 (Cell Signaling, Danvers, MA, USA), mouse antihuman AKT (Cell Signaling), rabbit antihuman p-Rictor Thr1135 (Cell Signaling), mouse antihuman Rictor (Santa Cruz Biotechnology; Dallas, TX, USA), rabbit antihuman p-mTOR Ser 2448 (Santa Cruz), rabbit antihuman mTOR (Cell Signaling), mouse antihuman p-4E-BP1 Ser 65 (Santa Cruz), mouse antihuman p-4E-BP1 (Santa Cruz), rabbit antihuman p-S6K1 Thr 421/Ser 424 (Santa Cruz), rabbit antihuman S6K1 (Cell Signaling), rabbit antihuman Bmi-1 (Cell Signaling). As surrogate for loading control we used Western blot performed with 1:100,000 antihuman β-actin antibody (Santa Cruz). Membranes were exposed to 1:10,000 affinity-purified secondary antibodies conjugated with horse-radish peroxidase (Jackson Laboratories; West Grove, PA, USA). Immunoreactive proteins were visualized by SuperSignal West Pico chemiluminescent substrate (Thermo Scientific).

Andrade N.P., Warner K.A., Zhang Z., Pearson A.T., Mantesso A., Guimaraēs D.M., Altemani A., Mariano F.V., Nunes F.D, & Nör J.E. (2021). Survival of salivary gland cancer stem cells requires mTOR signaling. Cell Death & Disease, 12(1), 108.

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Western Blot Analysis of LC3, mTOR, and GAPDH

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Western blotting analysis was performed according to the method described in the literature (Deng et al., 2016 (link)). The primary (rabbit anti-human LC3, rabbit anti-human mTOR and mouse anti-human GAPDH antibodies) and secondary (anti-rabbit horseradish peroxidase-labeled antibody and anti-mouse horseradish peroxidase-labeled antibody) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) and used at a dilution of 1:1,000 and 1:10,000, respectively. The results were quantified using ImageJ software.

Liu W.N., Jiang X.Y., Xu Y.Z., Sun X.H., Wu K.X., Hu X.L., Lin Y., Lin L.R., Tong M.L, & Liu L.L. (2020). Treponema pallidum Dysregulates Monocytes and Promotes the Expression of IL-1β and Migration in Monocytes Through the mTOR Signaling Pathway. Frontiers in Cellular and Infection Microbiology, 10, 592864.

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