Penicillin streptomycin liquid

Manufactured by Sangon

Penicillin-streptomycin liquid is a commonly used antibiotic solution for cell culture applications. It contains a combination of the antibiotics penicillin and streptomycin, which are effective against a broad spectrum of bacteria. This product is used to prevent bacterial contamination in cell culture media and experiments.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sangon (3 mentions) β mercaptoethanol, by Sangon (2 mentions) Dnase 1, by Merck Group (1 mentions) Collagenase 4, by Solarbio (1 mentions) Ly294002, by Solarbio (1 mentions)

Spelling variants of this product

Streptomycin (151 citations) Penicillin (150 citations) Penicillin/streptomycin (110 citations)

3 protocols using penicillin streptomycin liquid

Isolation and Culture of Skin and Spleen Cells

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Skin samples were cut into 0.5 cm × 0.5 cm pieces and incubated in the presence of 0.5% trypsin (cat. no. Y0002311; Sigma-Aldrich; Merck KGaA) at 37°C for 2 h. Upon separation of the epidermis, the dermis was digested with DNase I (cat. no. D8071; Beijing Solarbio Science & Technology Co., Ltd.) and collagenase IV (cat. no. C5138; Sigma-Aldrich; Merck KGaA) in DMEM (cat. no. E600008-0500; Sangon Biotech Co., Ltd.) for 37°C, 1 h. Cells were harvested and resuspended (1×10⁶ cells/ml) for use in subsequent flow cytometry analysis.
Spleen samples were triturated and filtered through cell strainers (70 and 40 µm; cat. no. F613462 and F613461; Sangon Biotech Co., Ltd.). Cell suspension was collected and treated according to the protocol of mouse splenic mononuclear cell isolation kit (cat. no. LDS1090PK; Tianjin Haoyang Biological Products Technology Co., Ltd.). Splenic mononuclear cells were acquired and suspended in RPMI-1640 medium (cat. no. E600028; Sangon Biotech Co., Ltd.) with 10% fetal bovine serum (cat. no. E510008-0500; Sangon Biotech Co., Ltd.), 1% penicillin-streptomycin liquid, 1% nonessential amino acid and 0.01% β-mercaptoethanol (cat. nos. P1400, N1250 and M8210, respectively; Beijing Solarbio Science & Technology Co., Ltd.). Then cells were counted and adjusted to 10⁶ cells/ml for subsequent treatment and experiments.

Lin Y.W., Li X.X., Fu F.H., Liu B., Xing X., Qi R, & Ma L. (2022). Notch1/Hes1-PTEN/AKT/IL-17A feedback loop regulates Th17 cell differentiation in mouse psoriasis-like skin inflammation. Molecular Medicine Reports, 26(1), 223.

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Psoriasis Peripheral Blood Mononuclear Cell Stimulation

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Serum samples were acquired from all psoriasis patients and healthy controls after an overnight fast in the morning between 8:00 and 9:00 a.m. Peripheral blood mononuclear cells (PBMCs) from 10 severity psoriasis patients were isolated and collected by Ficoll–Hypaque density gradient centrifugation, cultured at a cell concentration of 2 × 10⁶ cells/mL in RPMI 1640 medium (cat. no. E600028; Sangon Biotech108 Co., Ltd.) supplemented with 10% fetal bovine serum (cat. no. E510008‐0500; Sangon Biotech Co., Ltd.), and 1% penicillin–streptomycin liquid (cat. nos. P1400, Beijing Solarbio Science & Technology Co., Ltd.), then stimulated and cocultured with different concentrations of rHMGB1 (0, 10, 100, 200, and 400 ng/mL, cat. no. 557804; Biolegend), respectively at 37°C for 24 h in a humidified incubator with a 5% CO₂ for subsequent flow cytometric analysis, real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) and western blot detection. All the experiments were repeated three times.

Zhu X., Dou Y., Lin Y., Chu G., Wang J, & Ma L. (2024). HMGB1 regulates Th17 cell differentiation and function in patients with psoriasis. Immunity, Inflammation and Disease, 12(2), e1205.

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Splenic Mononuclear Cell Isolation and LY294002 Treatment

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Spleens were isolated under aseptic conditions. The splenic mononuclear cell suspension was prepared by grinding the spleens through 70 μm and 40 μm cell strainers (cat. no. F613462 and F613461; Sangon Biotech Co., Ltd., China). After lysing red blood cells by Sample Diluent (cat. no. 2010 C1119; Tianjin Haoyang Biological Products Technology Co., Ltd., China), splenic mononuclear cells were isolated, collected, and cultured in RPMI 1640 medium (cat. no. E600028; Sangon Biotech Co., Ltd.) supplemented with 10% fetal bovine serum (cat. no. E510008-0500; Sangon Biotech Co., Ltd.), 1% penicillin-streptomycin liquid, 1% nonessential amino acid, and 0.01% β-mercaptoethanol (cat. nos. P1400, N1250 and M8210, respectively; Beijing Solarbio Science & Technology Co., Ltd., China) at 37 °C in a humidified incubator with a 5% CO 2 .
Then, splenic mononuclear cells were counted and adjusted to 1 × 10 6 cells/ml with LY294002 treatment (50 μmol/l, dissolved in DMSO) for 0, 4, 8, 16, and 24 h respectively. In addition, the above cells were also treated by LY294002 (50 μmol/l) alone as well as LY294002 with chloroquine (CQ, 100 μmol/l dissolved in PBS, cat. no. C6628; Sigma-Aldrich; Merck KGaA) together for 24 h. To avoid any nonspecific toxic effects of DMSO on cell growth, DMSO concentration was maintained at 0.5% in all experiments.

Lin Y., Zhu X., Li Y., Dou Y., Wang J., Qi R, & Ma L. (2023). LY294002 ameliorates psoriatic skin inflammation in mice via blocking the Notch1/Hes1-PTEN/AKT/IL-17A feedback loop. Clinical and experimental immunology, 213(1).

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