Epidermal sheets were prepared and stained as previously described (Lewis et al., 2014 (link)) with anti-CD207 (RMUL.2, eBioscience), anti-TCRγδ (GL3, Becton Dickinson), anti-p53 (NCL-p53-CM5p, Leica Biosystems) followed by fluorescent species-specific secondaries (Jackson Immunoresearch). Z-stacked images (20/mouse) were collected (Zeiss 510Meta confocal) in a set pattern and Volocity 6.2 (Perkin Elmer, Waltham, MA) used to quantitate LC and DETC. CPD staining and quantification is described in Supplementary Figure S1 and p53 island quantification in Supplementary Figure S3 .
Anti p53 ncl p53 cm5p
Manufactured by Leica
Anti-p53 (NCL-p53-CM5p) is a mouse monoclonal antibody that recognizes the p53 protein. p53 is a tumor suppressor protein that plays a crucial role in regulating cell growth and division. This antibody can be used for the detection of p53 in various applications, such as immunohistochemistry and Western blotting.
Automatically generated - may contain errors
2 protocols using anti p53 ncl p53 cm5p
1
Epidermal Immune Cell Quantification
2
Epidermal Immune Cell Quantification
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